Cloning and characterization of sf9 cell lamin and the lamin conformational changes during autographa californica multiple nucleopolyhedrovirus infection
文献类型：期刊论文
| 作者 | Wei, Wenqiang1; Wang, Hongju1; Li, Xiaoya2; Fang, Na1; Yang, Shili3; Liu, Hongyan1; Kang, Xiaonan2; Sun, Xiulian3; Ji, Shaoping1 |
| 刊名 | Viruses-basel
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 8期号:5页码:10 |
| 关键词 | Autographa californica multiple nucleopolyhedrovirus Infection Sf9 cells Lamina |
| ISSN号 | 1999-4915 |
| DOI | 10.3390/v8050126 |
| 通讯作者 | Sun, xiulian(sunxl@wh.iov.cn) ; Ji, shaoping(bioscience123@hotmail.com) |
| 英文摘要 | At present, the details of lamina alterations after baculovirus infection remain elusive. in this study, a lamin gene in the sf9 cell line of spodoptera frugiperda was cloned. the open reading frame (orf) of the sf9 lamin was 1860 bp and encoded a protein with a molecular weight of 70 kda. a transfection assay with a red fluorescence protein (rfp)-lamin fusion protein indicated that sf9 lamin was localized in the nuclear rim. transmission electron microscopy observations indicated that autographa californica multiple nucleopolyhedrovirus (acmnpv) nucleocapsids may pass through the nuclear envelope. immunofluorescence assay indicated that the lamina showed a ruffled staining pattern with the formation of invaginations in the sf9 cells infected with acmnpv, while it was evenly distributed at the nuclear periphery of mock-infected cells. western blotting results indicated that the total amount of lamin in the baculovirus-infected sf9 cells was significantly decreased compared with the mock-infected cells. these results imply that acmnpv infection induces structural and biochemical rearrangements of lamina of sf9 cells. |
| WOS关键词 | INTRANUCLEAR MICROVESICLE FORMATION ; NUCLEAR POLYHEDROSIS-VIRUS ; SPODOPTERA-FRUGIPERDA ; MOLECULAR-BIOLOGY ; EFFICIENT EGRESS ; NUCLEOCAPSIDS ; ENVELOPE ; REPLICATION ; SEQUENCES ; RESPONSES |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000377429600009 |
| 出版者 | MDPI AG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373487 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Sun, Xiulian; Ji, Shaoping |
| 作者单位 | 1.Henan Univ, Sch Med, Lab Cell Signal Transduct, Kaifeng 475004, Peoples R China 2.Henan Univ, Sch Educ Sci, Kaifeng 475004, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Wenqiang,Wang, Hongju,Li, Xiaoya,et al. Cloning and characterization of sf9 cell lamin and the lamin conformational changes during autographa californica multiple nucleopolyhedrovirus infection[J]. Viruses-basel,2016,8(5):10. |
| APA | Wei, Wenqiang.,Wang, Hongju.,Li, Xiaoya.,Fang, Na.,Yang, Shili.,...&Ji, Shaoping.(2016).Cloning and characterization of sf9 cell lamin and the lamin conformational changes during autographa californica multiple nucleopolyhedrovirus infection.Viruses-basel,8(5),10. |
| MLA | Wei, Wenqiang,et al."Cloning and characterization of sf9 cell lamin and the lamin conformational changes during autographa californica multiple nucleopolyhedrovirus infection".Viruses-basel 8.5(2016):10. |
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来源：武汉病毒研究所
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