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Chinese Academy of Sciences Institutional Repositories Grid
Two-dimensional crystallization and preliminary structure analysis of lhc-ii from cucumber and spinach

文献类型:期刊论文

作者Xu, W; Zhang, X; Lou, SQ; Wang, KB; Huang, YG; Kuang, TY
刊名Science in china series c-life sciences
出版日期1998-06-01
卷号41期号:3页码:265-271
关键词Light-harvesting chlorophyll a/b protein complex Two-dimensional crystallization Electron microscopy Image processing Structure analysis Cucumber Spinach
ISSN号1006-9305
通讯作者Xu, w()
英文摘要Large and well-ordered two-dimensional (2d) crystals of the light-harvesting chlorophyll a/b protein complexes (lhc-ii) from cucumber and spinach chloroplasts were produced by the so-called batch method. the two-dimensional structures of these crystals were examined at about 1.5 nm resolution by electron microscopy and image processing. the projection maps showed that there were similar, but not identical, structure features between two different lhc-ii complexes. a comparison between 2d crystal formations of the two different lhc-ii complexes was done and some factors affecting 2d crystallization of the membrane proteins were analyzed. the relations of the structures of the lhc-ii complexes to their polypeptide components and chi a/b ratio were also discussed.
WOS关键词CHLOROPHYLL-A/B-PROTEIN ; LIGHT-HARVESTING COMPLEX ; 3-DIMENSIONAL STRUCTURE ; ELECTRON CRYSTALLOGRAPHY ; CRYSTALS ; MICROSCOPY ; MODEL
WOS研究方向Life Sciences & Biomedicine - Other Topics
WOS类目Biology
语种英语
WOS记录号WOS:000074448600007
出版者SCIENCE PRESS
URI标识http://www.irgrid.ac.cn/handle/1471x/2373824
专题物理研究所
通讯作者Xu, W
作者单位1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Beijing Lab Electron Microscopy, Ctr Condensed Matter Phys, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714
Xu, W,Zhang, X,Lou, SQ,et al. Two-dimensional crystallization and preliminary structure analysis of lhc-ii from cucumber and spinach[J]. Science in china series c-life sciences,1998,41(3):265-271.
APA Xu, W,Zhang, X,Lou, SQ,Wang, KB,Huang, YG,&Kuang, TY.(1998).Two-dimensional crystallization and preliminary structure analysis of lhc-ii from cucumber and spinach.Science in china series c-life sciences,41(3),265-271.
MLA Xu, W,et al."Two-dimensional crystallization and preliminary structure analysis of lhc-ii from cucumber and spinach".Science in china series c-life sciences 41.3(1998):265-271.

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