Revealing three stages of dna-cisplatin reaction by a solid-state nanopore
文献类型：期刊论文
| 作者 | Zhou, Zhi1,2; Hu, Ying1,2; Shan, Xinyan1,2; Li, Wei1,2; Bai, Xuedong1,2,3; Wang, Pengye1,2; Lu, Xinghua1,2,3 |
| 刊名 | Scientific reports
 |
| 出版日期 | 2015-07-07 |
| 卷号 | 5页码:9 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep11868 |
| 通讯作者 | Lu, xinghua(xhlu@iphy.ac.cn) |
| 英文摘要 | The dynamic structural behavior in dna due to interaction with cisplatin is essential for the functionality of platinum-based anti-cancer drugs. here we report a novel method to monitor the interaction progress in dna-cisplatin reaction in real time with a solid-state nanopore. the interaction processes are found to be well elucidated by the evolution of the capture rate of dna-cisplatin complex, which is defined as the number of their translocation events through the nanopore in unit time. in the first stage, the capture rate decreases rapidly due to dna discharging as the positive-charged hydrated cisplatin molecules initially bond to the negative-charged dna and form mono-adducts. in the second stage, by forming di-adducts, the capture rate increases as dna molecules are softened, appears as the reduced persistence length of the dna-cisplatin adducts. in the third stage, the capture rate decreases again as a result of dna aggregation. our study demonstrates a new single-molecule tool in exploring dynamic behaviors during drug-dna reactions and may have future application in fast drug screening. |
| WOS关键词 | METAL-COMPLEXES ; SINGLE ; MOLECULES ; ADDUCTS ; CANCER |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000357453300002 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374061 |
| 专题 | 物理研究所 |
| 通讯作者 | Lu, Xinghua |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Phys, Beijing 100190, Peoples R China 3.Collaborat Innovat Ctr Quantum Matter, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Zhi,Hu, Ying,Shan, Xinyan,et al. Revealing three stages of dna-cisplatin reaction by a solid-state nanopore[J]. Scientific reports,2015,5:9. |
| APA | Zhou, Zhi.,Hu, Ying.,Shan, Xinyan.,Li, Wei.,Bai, Xuedong.,...&Lu, Xinghua.(2015).Revealing three stages of dna-cisplatin reaction by a solid-state nanopore.Scientific reports,5,9. |
| MLA | Zhou, Zhi,et al."Revealing three stages of dna-cisplatin reaction by a solid-state nanopore".Scientific reports 5(2015):9. |
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来源：物理研究所
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