Bacterial diversity in native habitats of the medicinal fungus ophiocordyceps sinensis on tibetan plateau as determined using illumina sequencing data
文献类型:期刊论文
作者 | Yang, Rui-Heng1,2,6; Wang, Xiao-Liang1,2; Su, Jin-He3,6; Li, Yi1,2,6; Jiang, Si-Ping2,4; Gu, Fei5; Yao, Yi-Jian1,2 |
刊名 | Fems microbiology letters
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出版日期 | 2015-03-01 |
卷号 | 362期号:5页码:8 |
关键词 | Bacterial diversity Illumina deep sequencing technology Ophiocordyceps sinensis Soil 16s rrna |
ISSN号 | 0378-1097 |
DOI | 10.1093/femsle/fnu044 |
通讯作者 | Yao, yi-jian(yaoyj@im.ac.cn) |
英文摘要 | Ophiocordyceps sinensis is one of the most well-known traditional chinese medicinal fungi. in this study, bacterial diversity in the soils of native habitats of o. sinensis was investigated using illumina sequencing data. a total of 525 000 sequences of v6-16s rrna were analyzed. the number of otus from each sample ranged from 13 858 to 15 978 at 97% sequence similarity cut-off. the results demonstrated that the deep sequencing approach provides improved access to rare genotypes. richness indices and shannon's diversity index did not differ significantly between samples collected from locations where o. sinensis was present (os1-3) and not present (nos1-3). classified bacterial sequences were grouped into 23 phyla including proteobacteria, actinobacteria, acidobacteria, verrucomicrobia, etc. the venn diagram revealed that 7183 otus belonging to 14 phyla were shared by os, nos and mp (mycelial pellicle wrapping the sclerotium of o. sinensis) samples, possibly representing a core microbiome existing in native habitats of o. sinensis, and that 863 belonging to 12 phyla were shared by os and mp samples, possibly related to the occurrence of o. sinensis. overall, the results revealed a high bacterial diversity in the soil samples and the relationships between the bacterial diversity and o. sinensis merit further investigation. |
WOS关键词 | SOIL MICROBIAL COMMUNITIES ; GIGASPORA-MARGARITA ; BIOSPHERE ; PATTERNS ; SPORES ; PLANTS ; LAKE |
WOS研究方向 | Microbiology |
WOS类目 | Microbiology |
语种 | 英语 |
WOS记录号 | WOS:000356885500005 |
出版者 | OXFORD UNIV PRESS |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374079 |
专题 | 计算机网络信息中心 |
通讯作者 | Yao, Yi-Jian |
作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China 2.ITPB IMCAS Key Joint Lab Alpine Fungi, Lhasa 850001, Peoples R China 3.Chinese Acad Sci, Comp Network Informat Ctr, Beijing 100190, Peoples R China 4.Tibet Plateau Inst Biol, Lhasa 850001, Peoples R China 5.Aba Acad Sci & Technol, Inst Anim Sci, Wenchuan 623000, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, Rui-Heng,Wang, Xiao-Liang,Su, Jin-He,et al. Bacterial diversity in native habitats of the medicinal fungus ophiocordyceps sinensis on tibetan plateau as determined using illumina sequencing data[J]. Fems microbiology letters,2015,362(5):8. |
APA | Yang, Rui-Heng.,Wang, Xiao-Liang.,Su, Jin-He.,Li, Yi.,Jiang, Si-Ping.,...&Yao, Yi-Jian.(2015).Bacterial diversity in native habitats of the medicinal fungus ophiocordyceps sinensis on tibetan plateau as determined using illumina sequencing data.Fems microbiology letters,362(5),8. |
MLA | Yang, Rui-Heng,et al."Bacterial diversity in native habitats of the medicinal fungus ophiocordyceps sinensis on tibetan plateau as determined using illumina sequencing data".Fems microbiology letters 362.5(2015):8. |
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