An unexpected similarity between antibiotic-resistant ndm-1 and beta-lactamase ii from erythrobacter litoralis
文献类型：期刊论文
| 作者 | Zheng, Beiwen1,2; Tan, Shuguang1,2; Gao, Jia1,3; Han, Huiming1,2; Liu, Jun1; Lu, Guangwen1,2; Liu, Di1,4; Yi, Yong; Zhu, Baoli1; Gao, George F.1,2,5 |
| 刊名 | Protein & cell
 |
| 出版日期 | 2011-03-01 |
| 卷号 | 2期号:3页码:250-258 |
| 关键词 | Ndm-1 Metallo-beta-lactamase Erythrobacter litoralis Similarity Antibiotics resistance |
| ISSN号 | 1674-800X |
| DOI | 10.1007/s13238-011-1027-0 |
| 通讯作者 | Gao, george f.(gaof@im.ac.cn) |
| 英文摘要 | Ndm-1 (new delhi metallo-beta-lactamase) gene encodes a metallo-beta-lactamase (mbl) with high carbapenemase activity, which makes the host bacterial strain easily dispatch the last-resort antibiotics known as carbapenems and cause global concern. here we present the bioinformatics data showing an unexpected similarity between ndm-1 and beta-lactamase ii from erythrobacter litoralis, a marine microbial isolate. we have further expressed these two mature proteins in e. coli cells, both of which present as a monomer with a molecular mass of 25 kda. antimicrobial susceptibility assay reveals that they share similar substrate specificities and are sensitive to aztreonam and tigecycline. the conformational change accompanied with the zinc binding visualized by nuclear magnetic resonance, zn2+-bound ndm-1, adopts at least some stable tertiary structure in contrast to the metal-free protein. our work implies a close evolutionary relationship between antibiotic resistance genes in environmental reservoir and in the clinic, challenging the antimicrobial resistance monitoring. |
| WOS关键词 | NATURAL ENVIRONMENTS ; GENE-TRANSFER ; SP-NOV ; ENTEROBACTERIACEAE ; CARBAPENEMASES ; EMERGENCE ; WORLDWIDE ; BINDING ; INDIA |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000310519700009 |
| 出版者 | HIGHER EDUCATION PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374137 |
| 专题 | 计算机网络信息中心 |
| 通讯作者 | Gao, George F. |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing 100101, Peoples R China 3.Guangxi Univ, Key Lab Minist Educ Microbial & Plant Genet Engn, Nanning 530004, Peoples R China 4.Chinese Acad Sci, Inst Microbiol, Network Informat Ctr, Beijing 100101, Peoples R China 5.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Beiwen,Tan, Shuguang,Gao, Jia,et al. An unexpected similarity between antibiotic-resistant ndm-1 and beta-lactamase ii from erythrobacter litoralis[J]. Protein & cell,2011,2(3):250-258. |
| APA | Zheng, Beiwen.,Tan, Shuguang.,Gao, Jia.,Han, Huiming.,Liu, Jun.,...&Gao, George F..(2011).An unexpected similarity between antibiotic-resistant ndm-1 and beta-lactamase ii from erythrobacter litoralis.Protein & cell,2(3),250-258. |
| MLA | Zheng, Beiwen,et al."An unexpected similarity between antibiotic-resistant ndm-1 and beta-lactamase ii from erythrobacter litoralis".Protein & cell 2.3(2011):250-258. |
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