High-throughput analysis of the t cell receptor beta chain repertoire in pbmcs from chronic hepatitis b patients with hbeag seroconversion
文献类型：期刊论文
| 作者 | Qu, Yachao1; Huang, Yong2; Liu, Di3; Huang, Yinuo1; Zhang, Zhiyi2; Mi, Zhiqiang2; An, Xiaoping2; Tong, Yigang2; Lu, Jun1 |
| 刊名 | Canadian journal of infectious diseases & medical microbiology
 |
| 出版日期 | 2016 |
| 页码 | 7 |
| ISSN号 | 1712-9532 |
| DOI | 10.1155/2016/8594107 |
| 通讯作者 | Tong, yigang(tong.yigang@gmail.com) ; Lu, jun(lujun98@ccmu.edu.cn) |
| 英文摘要 | T lymphocytes are the most important immune cells that affect both the development and treatment of hepatitis b. we used high-throughput sequencing to determine the diversity in the v and j regions of the tcr beta. chain in 4 chronic hepatitis b patients before and after hbeag seroconversion. here, we demonstrate that the 4 patients expressed v beta 12-4 at the highest frequencies of 10.6%, 9.2%, 17.5%, and 7.5%, and v beta 28 was the second most common, with frequencies of 7.8%, 6.7%, 5.3%, and 10.9%, respectively. no significant changes were observed following seroconversion. with regard to the j beta gene, j beta 2-1 was the most commonly expressed in the 4 patients at frequencies of 5.8%, 6.5%, 11.3%, and 7.3%, respectively. analysis of the v-j region genes revealed several differences, including significant increases in the expression levels of v7-2-01-j2-1, v12-4-j1-1, and v28-1-j1-5 and a decrease in that of v19-01-j2-3. these results illustrate the presence of biased tcrv beta and j beta gene expression in the chronic hepatitis b patients. trbv beta 12-4, v beta 28, j beta 2-1, v7-2-01-j2-1, v12-4-j1-1, and v28-1-j1-5 may be associated with the development and treatment of chb. |
| WOS关键词 | ANTIGEN RECEPTOR ; AUTOIMMUNE HEPATITIS ; IMMUNE-RESPONSE ; CDR3 LENGTH ; VIRUS ; LYMPHOCYTES ; DISEASE ; USAGE ; LIVER ; REARRANGEMENT |
| WOS研究方向 | Infectious Diseases ; Microbiology |
| WOS类目 | Infectious Diseases ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000386271000001 |
| 出版者 | HINDAWI PUBLISHING CORP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374201 |
| 专题 | 计算机网络信息中心 |
| 通讯作者 | Tong, Yigang; Lu, Jun |
| 作者单位 | 1.Capital Med Univ, Beijing Youan Hosp, Hepatol & Canc Biotherapy Ward, Beijing, Peoples R China 2.Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Network Informat Ctr, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Yachao,Huang, Yong,Liu, Di,et al. High-throughput analysis of the t cell receptor beta chain repertoire in pbmcs from chronic hepatitis b patients with hbeag seroconversion[J]. Canadian journal of infectious diseases & medical microbiology,2016:7. |
| APA | Qu, Yachao.,Huang, Yong.,Liu, Di.,Huang, Yinuo.,Zhang, Zhiyi.,...&Lu, Jun.(2016).High-throughput analysis of the t cell receptor beta chain repertoire in pbmcs from chronic hepatitis b patients with hbeag seroconversion.Canadian journal of infectious diseases & medical microbiology,7. |
| MLA | Qu, Yachao,et al."High-throughput analysis of the t cell receptor beta chain repertoire in pbmcs from chronic hepatitis b patients with hbeag seroconversion".Canadian journal of infectious diseases & medical microbiology (2016):7. |
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