Chromatin accessibility dynamics during chemical induction of pluripotency
文献类型：期刊论文
| 作者 | Cao, Shangtao1,2,4; Yu, Shengyong1,2; Li, Dongwei1,2; Ye, Jing1,2; Yang, Xuejie1,2,4; Li, Chen1,2,4; Wang, Xiaoshan1,2,3,4; Mai, Yuanbang1,2; Qin, Yue1,2,4; Wu, Jian1,2,4 |
| 刊名 | Cell stem cell
 |
| 出版日期 | 2018-04-05 |
| 卷号 | 22期号:4页码:529-+ |
| ISSN号 | 1934-5909 |
| DOI | 10.1016/j.stem.2018.03.005 |
| 通讯作者 | Liu, jing(liu_jing@gibh.ac.cn) ; Pei, duanqing(pei_duanqing@gibh.ac.cn) |
| 英文摘要 | Despite its exciting potential, chemical induction of pluripotency (cip) efficiency remains low and the mechanisms are poorly understood. we report the development of an efficient two-step serum-and replating-free cip protocol and the associated chromatin accessibility dynamics (cad) by assay for transposase-accessible chromatin (atac)-seq. cip reorganizes the somatic genome to an intermediate state that is resolved under 2il condition by reclosing previously opened loci prior to pluripotency acquisition with gradual opening of loci enriched with motifs for the oct/sox/klf families. bromodeoxyuridine, a critical ingredient of cip, is responsible for both closing and opening critical loci, at least in part by preventing the opening of loci enriched with motifs for the ap1 family and facilitating the opening of loci enriched with sox/klf/gata motifs. these changes differ markedly from cad observed during yamanaka-factor-driven reprogramming. our study provides insights into small-molecule-based reprogramming mechanisms and reorganization of nuclear architecture associated with cell-fate decisions. |
| WOS关键词 | SMALL-MOLECULE COMPOUNDS ; STEM-CELLS ; SOMATIC-CELLS ; REGENERATIVE MEDICINE ; MOUSE FIBROBLASTS ; DEFINED FACTORS ; GROUND-STATE ; OCT4 ; GENERATION ; VIRUS |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell & Tissue Engineering ; Cell Biology |
| 语种 | 英语 |
| 出版者 | CELL PRESS |
| WOS记录号 | WOS:000429320900012 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374246 |
| 专题 | 计算机网络信息中心 |
| 通讯作者 | Liu, Jing; Pei, Duanqing |
| 作者单位 | 1.Chinese Acad Sci, South China Inst Stem Cell Biol & Regenerat Med, Guangzhou Inst Biomed & Hlth, CAS Key Lab Regenerat Biol, Guangzhou 510530, Guangdong, Peoples R China 2.Chinese Acad Sci, South China Inst Stem Cell Biol & Regenerat Med, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangzhou 510530, Guangdong, Peoples R China 3.Chinese Acad Sci, Supercomp Ctr, Guangzhou Branch, Guangzhou 510530, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.South China Univ Technol, Sch Med, Guangzhou 510006, Guangdong, Peoples R China 6.Chinese Univ Hong Kong, CUHK GIBH Joint Lab Stem Cell & Regenerat Med, Shatin, Hong Kong, Peoples R China 7.GUANGZHOU Regenerat Med & Hlth Guangdong Lab GIBH, Guangzhou 510530, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Shangtao,Yu, Shengyong,Li, Dongwei,et al. Chromatin accessibility dynamics during chemical induction of pluripotency[J]. Cell stem cell,2018,22(4):529-+. |
| APA | Cao, Shangtao.,Yu, Shengyong.,Li, Dongwei.,Ye, Jing.,Yang, Xuejie.,...&Pei, Duanqing.(2018).Chromatin accessibility dynamics during chemical induction of pluripotency.Cell stem cell,22(4),529-+. |
| MLA | Cao, Shangtao,et al."Chromatin accessibility dynamics during chemical induction of pluripotency".Cell stem cell 22.4(2018):529-+. |
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