Single-cell rna sequencing reveals dynamic changes in a-to-i rna editome during early human embryogenesis
文献类型：期刊论文
| 作者 | Qiu,Si1,2; Li,Wenhui2; Xiong,Heng2; Liu,Dongbing2; Bai,Yali2,3; Wu,Kui2,4; Zhang,Xiuqing2; Yang,Huanming2,5; Ma,Kun2; Hou,Yong2,4 |
| 刊名 | Bmc genomics
 |
| 出版日期 | 2016-09-29 |
| 卷号 | 17期号:1 |
| 关键词 | Rna-editing Single cell transcriptome Embryogenesis |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/s12864-016-3115-2 |
| 通讯作者 | Ma,kun(makun1@genomics.cn) ; Hou,yong(houyong@genomics.cn) ; Li,bo(libo@genomics.cn) |
| 英文摘要 | Abstractbackgrounda-to-i rna-editing mediated by adar (adenosine deaminase acting on rna) enzymes that converts adenosine to inosine in rna sequence can generate mutations and alter gene regulation in metazoans. previous studies have shown that a-to-i rna-editing plays vital roles in mouse embryogenesis. however, the rna-editing activities in early human embryonic development have not been investigated.resultshere, we characterized genome-wide a-to-i rna-editing activities during human early embryogenesis by profiling 68 single cells from 29 human embryos spanning from oocyte to morula stages. we demonstrate dynamic changes in genome-wide rna-editing during early human embryogenesis in a stage-specific fashion. in parallel with adar expression level changes, the genome-wide a-to-i rna-editing levels in cells remained relatively stable until 4-cell stage, but dramatically decreased at 8-cell stage, continually decreased at morula stage. we detected 37 non-synonymously rna-edited genes, of which 5 were frequently found in cells of multiple embryonic stages. moreover, we found that a-to-i editings in mirna-targeted regions of a substantial number of genes preferably occurred in one or two sequential stages.conclusionsour single-cell analysis reveals dynamic changes in genome-wide rna-editing during early human embryogenesis in a stage-specific fashion, and provides important insights into early human embryogenesis. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/S12864-016-3115-2 |
| 出版者 | BioMed Central |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374279 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ma,Kun; Hou,Yong; Li,Bo |
| 作者单位 | 1.University of Chinese Academy of Sciences; BGI Education Center 2.BGI-Shenzhen 3.Huazhong University of Science and Technology; College of Life Science and Technology 4.University of Copenhagen; Department of Biology 5.James D. Watson Institute of Genome Sciences 6.BGI-Forensics |
| 推荐引用方式 GB/T 7714 | Qiu,Si,Li,Wenhui,Xiong,Heng,et al. Single-cell rna sequencing reveals dynamic changes in a-to-i rna editome during early human embryogenesis[J]. Bmc genomics,2016,17(1). |
| APA | Qiu,Si.,Li,Wenhui.,Xiong,Heng.,Liu,Dongbing.,Bai,Yali.,...&Li,Bo.(2016).Single-cell rna sequencing reveals dynamic changes in a-to-i rna editome during early human embryogenesis.Bmc genomics,17(1). |
| MLA | Qiu,Si,et al."Single-cell rna sequencing reveals dynamic changes in a-to-i rna editome during early human embryogenesis".Bmc genomics 17.1(2016). |
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来源：中国科学院大学
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