Itis, a bioinformatics tool for accurate identification of transposon insertion sites using next-generation sequencing data
文献类型：期刊论文
| 作者 | Jiang,Chuan1,2; Chen,Chao1,2; Huang,Ziyue1; Liu,Renyi1; Verdier,Jerome1 |
| 刊名 | Bmc bioinformatics
 |
| 出版日期 | 2015-03-05 |
| 卷号 | 16期号:1 |
| 关键词 | Transposable elements Insertion sites Itis Forward genetics Tnt1 Medicago truncatula |
| ISSN号 | 1471-2105 |
| DOI | 10.1186/s12859-015-0507-2 |
| 通讯作者 | Liu,renyi(ryliu@sibs.ac.cn) ; Verdier,jerome(javerdier@sibs.ac.cn) |
| 英文摘要 | Abstractbackgroundtransposable elements constitute an important part of the genome and are essential in adaptive mechanisms. transposition events associated with phenotypic changes occur naturally or are induced in insertional mutant populations. transposon mutagenesis results in multiple random insertions and recovery of most/all the insertions is critical for forward genetics study. using genome next-generation sequencing data and appropriate bioinformatics tool, it is plausible to accurately identify transposon insertion sites, which could provide candidate causal mutations for desired phenotypes for further functional validation.resultswe developed a novel bioinformatics tool, itis (identification of transposon insertion sites), for localizing transposon insertion sites within a genome. it takes next-generation genome re-sequencing data (ngs data), transposon sequence, and reference genome sequence as input, and generates a list of highly reliable candidate insertion sites as well as zygosity information of each insertion. using a simulated dataset and a case study based on an insertional mutant line from medicago truncatula, we showed that itis performed better in terms of sensitivity and specificity than other similar algorithms such as relocate, retroseq, temp and tif. with the case study data, we demonstrated the efficiency of itis by validating the presence and zygosity of predicted insertion sites of the tnt1 transposon within a complex plant system, m. truncatula.conclusionthis study showed that itis is a robust and powerful tool for forward genetic studies in identifying transposable element insertions causing phenotypes. itis is suitable in various systems such as cell culture, bacteria, yeast, insect, mammal and plant. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/S12859-015-0507-2 |
| 出版者 | BioMed Central |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374289 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu,Renyi; Verdier,Jerome |
| 作者单位 | 1.Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2.University of Chinese Academy of Sciences |
| 推荐引用方式 GB/T 7714 | Jiang,Chuan,Chen,Chao,Huang,Ziyue,et al. Itis, a bioinformatics tool for accurate identification of transposon insertion sites using next-generation sequencing data[J]. Bmc bioinformatics,2015,16(1). |
| APA | Jiang,Chuan,Chen,Chao,Huang,Ziyue,Liu,Renyi,&Verdier,Jerome.(2015).Itis, a bioinformatics tool for accurate identification of transposon insertion sites using next-generation sequencing data.Bmc bioinformatics,16(1). |
| MLA | Jiang,Chuan,et al."Itis, a bioinformatics tool for accurate identification of transposon insertion sites using next-generation sequencing data".Bmc bioinformatics 16.1(2015). |
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来源：中国科学院大学
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