Genome sequencing of high-penicillin producing industrial strain of penicillium chrysogenum
文献类型：期刊论文
| 作者 | Wang,Fu-Qiang1; Zhong,Jun2,3; Zhao,Ying1; Xiao,Jingfa2; Liu,Jing1; Dai,Meng1; Zheng,Guizhen1; Zhang,Li1; Yu,Jun2; Wu,Jiayan2 |
| 刊名 | Bmc genomics
 |
| 出版日期 | 2014-01-24 |
| 卷号 | 15期号:Suppl 1 |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/1471-2164-15-s1-s11 |
| 通讯作者 | Wu,jiayan(18603113322@163.com) ; Duan,baoling(wujy@big.ac.cn) |
| 英文摘要 | Abstractbackgrounddue to the importance of penicillium chrysogenum holding in medicine, the genome of low-penicillin producing laboratorial strain wisconsin54-1255 had been sequenced and fully annotated. through classical mutagenesis of wisconsin54-1255, product titers and productivities of penicillin have dramatically increased, but what underlying genome structural variations is still little known. therefore, genome sequencing of a high-penicillin producing industrial strain is very meaningful.resultsto reveal more insights into the genome structural variations of high-penicillin producing strain, we sequenced an industrial strain p. chrysogenum ncpc10086. by whole genome comparative analysis, we observed a large number of mutations, insertions and deletions, and structural variations. there are 69 new genes that not exist in the genome sequence of wisconsin54-1255 and some of them are involved in energy metabolism, nitrogen metabolism and glutathione metabolism. most importantly, we discovered a 53.7 kb "new shift fragment" in a seven copies of determinative penicillin biosynthesis cluster in ncpc10086 and the arrangement type of amplified region is unique. moreover, we presented two large-scale translocations in ncpc10086, containing genes involved energy, nitrogen metabolism and peroxysome pathway. at last, we found some non-synonymous mutations in the genes participating in homogentisate pathway or working as regulators of penicillin biosynthesis.conclusionswe provided the first high-quality genome sequence of industrial high-penicillin strain of p. chrysogenum and carried out a comparative genome analysis with a low-producing experimental strain. the genomic variations we discovered are related with energy metabolism, nitrogen metabolism and so on. these findings demonstrate the potential information for insights into the high-penicillin yielding mechanism and metabolic engineering in the future. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/1471-2164-15-S1-S11 |
| 出版者 | BioMed Central |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374301 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wu,Jiayan; Duan,Baoling |
| 作者单位 | 1.Hebei Industry Microbial Metabolic Engineering & Technology Research Center; New Drug Research and Development Center of North China Pharmaceutical Group Corporation, National Engineering Research Center of Microbial Medicine 2.Chinese Academy of Sciences; CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics 3.University of Chinese Academy of Sciences |
| 推荐引用方式 GB/T 7714 | Wang,Fu-Qiang,Zhong,Jun,Zhao,Ying,et al. Genome sequencing of high-penicillin producing industrial strain of penicillium chrysogenum[J]. Bmc genomics,2014,15(Suppl 1). |
| APA | Wang,Fu-Qiang.,Zhong,Jun.,Zhao,Ying.,Xiao,Jingfa.,Liu,Jing.,...&Duan,Baoling.(2014).Genome sequencing of high-penicillin producing industrial strain of penicillium chrysogenum.Bmc genomics,15(Suppl 1). |
| MLA | Wang,Fu-Qiang,et al."Genome sequencing of high-penicillin producing industrial strain of penicillium chrysogenum".Bmc genomics 15.Suppl 1(2014). |
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来源：中国科学院大学
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