Sabr enhances nikkomycin production via regulating the transcriptional level of sang, a pathway-specific regulatory gene in streptomyces ansochromogenes
文献类型：期刊论文
| 作者 | Pan,Yuanyuan1; Wang,Linqi2; He,Xihong1; Tian,Yuqing2; Liu,Gang1; Tan,Huarong2 |
| 刊名 | Bmc microbiology
 |
| 出版日期 | 2011-07-20 |
| 卷号 | 11期号:1 |
| ISSN号 | 1471-2180 |
| DOI | 10.1186/1471-2180-11-164 |
| 通讯作者 | Liu,gang(liug@sun.im.ac.cn) ; Tan,huarong(tanhr@im.ac.cn) |
| 英文摘要 | Abstractbackgroundsabr is a pleiotropic regulatory gene which has been shown to positively regulate the nikkomycin biosynthesis and negatively affect the sporulation of streptomyces ansochromogenes. in this study, we investigate the mechanism of sabr on modulating nikkomycin production in streptomyces ansochromogenes.resultsthe transcription start point of sabr was determined by high-resolution s1 nuclease mapping and localized at the nucleotide t at position 37 bp upstream of the potential sabr translation start codon (gtg). disruption of sabr enhanced its own transcription, but retarded the nikkomycin production. over-expression of sabr enhanced nikkomycin biosynthesis in streptomyces ansochromogenes. emsa analysis showed that sabr bound to the upstream region of sang, but it did not bind to the upstream region of its encoding gene (sabr), sanf and the intergenic region between sann and sano. dnase 1 footprinting assays showed that the sabr-binding site upstream of sang was 5'-ctttaagtcacctggctcattcgcgttcgcccagct-3' which was designated as sare. deletion of sare resulted in the delay of nikkomycin production that was similar to that of sabr disruption mutant.conclusionsthese results indicated that sabr modulated nikkomycin biosynthesis as an enhancer via interaction with the promoter region of sang, and expanded our understanding about regulatory cascade in nikkomycin biosynthesis. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/1471-2180-11-164 |
| 出版者 | BioMed Central |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374307 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu,Gang; Tan,Huarong |
| 作者单位 | 1.Chinese Academy of Sciences; The Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology 2.Chinese Academy of Sciences; State Key Laboratory of Microbial Resources, Institute of Microbiology |
| 推荐引用方式 GB/T 7714 | Pan,Yuanyuan,Wang,Linqi,He,Xihong,et al. Sabr enhances nikkomycin production via regulating the transcriptional level of sang, a pathway-specific regulatory gene in streptomyces ansochromogenes[J]. Bmc microbiology,2011,11(1). |
| APA | Pan,Yuanyuan,Wang,Linqi,He,Xihong,Tian,Yuqing,Liu,Gang,&Tan,Huarong.(2011).Sabr enhances nikkomycin production via regulating the transcriptional level of sang, a pathway-specific regulatory gene in streptomyces ansochromogenes.Bmc microbiology,11(1). |
| MLA | Pan,Yuanyuan,et al."Sabr enhances nikkomycin production via regulating the transcriptional level of sang, a pathway-specific regulatory gene in streptomyces ansochromogenes".Bmc microbiology 11.1(2011). |
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来源：中国科学院大学
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