Identification and characterization of the glucose dual-affinity transport system in neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression
文献类型：期刊论文
| 作者 | Wang,Bang1,2,3; Li,Jingen1; Gao,Jingfang1,4; Cai,Pengli1; Han,Xiaoyun4; Tian,Chaoguang1 |
| 刊名 | Biotechnology for biofuels
 |
| 出版日期 | 2017-01-19 |
| 卷号 | 10期号:1 |
| 关键词 | Glucose transporter Dual-affinity Glt-1 Hgt-1/-2 Cellulase Signaling |
| ISSN号 | 1754-6834 |
| DOI | 10.1186/s13068-017-0705-4 |
| 通讯作者 | Tian,chaoguang(tian_cg@tib.cas.cn) |
| 英文摘要 | Abstractbackgroundthe glucose dual-affinity transport system (low- and high-affinity) is a conserved strategy used by microorganisms to cope with natural fluctuations in nutrient availability in the environment. the glucose-sensing and uptake processes are believed to be tightly associated with cellulase expression regulation in cellulolytic fungi. however, both the identities and functions of the major molecular components of this evolutionarily conserved system in filamentous fungi remain elusive. here, we systematically identified and characterized the components of the glucose dual-affinity transport system in the model fungus neurospora crassa.resultsusing rna sequencing coupled with functional transport analyses, we assigned glt-1 (km?=?18.42?±?3.38?mm) and hgt-1/-2 (km?=?16.13?±?0.95 and 98.97?±?22.02?μm) to the low- and high-affinity glucose transport systems, respectively. the high-affinity transporters hgt-1/-2 complemented a moderate growth defect under high glucose when glt-1 was deleted. simultaneous deletion of hgt-1/-2 led to extensive derepression of genes for plant cell wall deconstruction in cells grown on cellulose. the suppression by hgt-1/-2 was connected to both carbon catabolite repression (ccr) and the cyclic adenosine monophosphate-protein kinase a pathway. alteration of a residue conserved across taxa in hexose transporters resulted in a loss of glucose-transporting function, whereas ccr signal transduction was retained, indicating dual functions for hgt-1/-2 as “transceptors.”conclusionsin this study, glt-1 and hgt-1/-2 were identified as the key components of the glucose dual-affinity transport system, which plays diverse roles in glucose transport and carbon metabolism. given the wide conservation of the glucose dual-affinity transport system across fungal species, the identification of its components and their pleiotropic roles in this study shed important new light on the molecular basis of nutrient transport, signaling, and plant cell wall degradation in fungi. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/S13068-017-0705-4 |
| 出版者 | BioMed Central |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374343 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tian,Chaoguang |
| 作者单位 | 1.Chinese Academy of Sciences; Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology 2.University of Chinese Academy of Sciences 3.Wenzhou Medical University; School of Ophthalmology and Optometry, Eye Hospital, State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry 4.Heilongjiang University; School of Life Sciences |
| 推荐引用方式 GB/T 7714 | Wang,Bang,Li,Jingen,Gao,Jingfang,et al. Identification and characterization of the glucose dual-affinity transport system in neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression[J]. Biotechnology for biofuels,2017,10(1). |
| APA | Wang,Bang,Li,Jingen,Gao,Jingfang,Cai,Pengli,Han,Xiaoyun,&Tian,Chaoguang.(2017).Identification and characterization of the glucose dual-affinity transport system in neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression.Biotechnology for biofuels,10(1). |
| MLA | Wang,Bang,et al."Identification and characterization of the glucose dual-affinity transport system in neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression".Biotechnology for biofuels 10.1(2017). |
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来源：中国科学院大学
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