中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Improving cellulase production in submerged fermentation by the expression of a vitreoscilla hemoglobin in trichoderma reesei

文献类型:期刊论文

作者Lin,Jie1,2; Zhang,Xiamei1,2; Song,Bingran1,2; Xue,Wei1; Su,Xiaoyun3; Chen,Xiuzhen1; Dong,Zhiyang1,2
刊名Amb express
出版日期2017-11-15
卷号7期号:1
ISSN号2191-0855
关键词Trichoderma reesei Vitreoscilla hemoglobin Submerged fermentation Oxygen limitation Cellulase
DOI10.1186/s13568-017-0507-x
通讯作者Chen,xiuzhen(chenxiuzhen@im.ac.cn) ; Dong,zhiyang(dongzy@im.ac.cn)
英文摘要Abstracttrichoderma reesei is well known as an industrial workhorse fungus in cellulase production. the low dissolved oxygen supply in the highly viscous medium of t. reesei remains a major bottleneck that hampers growth and cellulase production in submerged fermentation. vitreoscilla hemoglobin (vhb) has been demonstrated to improve metabolism and protein production in different heterologous hosts under hypoxic conditions, but the use of vhb in t. reesei remains uninvestigated. this study examines the effect of vhb in improving t. reesei performance in submerged fermentation. the vhb gene (vgb)-expressing cassette was successfully transformed into the tu-6 strain, integrated into the genome of t. reesei, and functionally expressed with biological activity, which was confirmed by carbon monoxide difference analysis. compared to the parent strain, the expression of vhb increased the glucose consumption rate of the transformant. moreover, in cellulase-inducing medium total protein secretion of the vhb expressing strain was 2.2-fold of the parental strain and the filter paper cellulase activity was increased by 58% under oxygen-limiting conditions. in summary, our results demonstrate that vhb has beneficial effects on improving total protein secretion and cellulase activity of t. reesei in submerged fermentation.
语种英语
出版者Springer Berlin Heidelberg
WOS记录号BMC:10.1186/S13568-017-0507-X
URI标识http://www.irgrid.ac.cn/handle/1471x/2374357
专题中国科学院大学
通讯作者Chen,Xiuzhen; Dong,Zhiyang
作者单位1.
2.
3.
推荐引用方式
GB/T 7714
Lin,Jie,Zhang,Xiamei,Song,Bingran,et al. Improving cellulase production in submerged fermentation by the expression of a vitreoscilla hemoglobin in trichoderma reesei[J]. Amb express,2017,7(1).
APA Lin,Jie.,Zhang,Xiamei.,Song,Bingran.,Xue,Wei.,Su,Xiaoyun.,...&Dong,Zhiyang.(2017).Improving cellulase production in submerged fermentation by the expression of a vitreoscilla hemoglobin in trichoderma reesei.Amb express,7(1).
MLA Lin,Jie,et al."Improving cellulase production in submerged fermentation by the expression of a vitreoscilla hemoglobin in trichoderma reesei".Amb express 7.1(2017).

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来源:中国科学院大学

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