Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells
文献类型：期刊论文
| 作者 | Xie, Yajun1,2; Wang, Jinlong1,2; Zhang, Yuanya1,2; Liu, Xiaofei1,2; Wang, Xiaorong1,2; Liu, Kehui1; Huang, Xiahe1; Wang, Yingchun1 |
| 刊名 | Scientific reports
 |
| 出版日期 | 2016-08-24 |
| 卷号 | 6页码:10 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep31811 |
| 通讯作者 | Wang, yingchun(ycwang@genetics.ac.cn) |
| 英文摘要 | Protein tyrosine phosphorylation is an important mechanism that regulates cytoskeleton reorganization and cell spreading of migratory cells. a number of cytoskeletal proteins are known to be tyrosine phosphorylated (py) in different cellular processes. however, the profile of py proteins during different stages of cell spreading has not been available. using immunoafffinity enrichment of py proteins coupled with label free quantitative proteomics, we quantitatively identified 447 py proteins in the migratory ecv-304 cells at the early spreading (adhesion) and the active spreading stages. we found that py levels of the majority of the quantified proteins were significantly increased in the active spreading stage compared with the early spreading stage, suggesting that active cell spreading is concomitant with extra tyrosine phosphorylation. the major categories of proteins impacted by tyrosine phosphorylation are involved in cytoskeleton and focal adhesion regulation, protein translation and degradation. our findings, for the first time, dissect the cell spreading-specific py signals from the adhesion induced py signals, and provide a valuable resource for the future mechanistic research regarding the regulation of cell spreading. |
| WOS关键词 | CANCER-CELLS ; MYOSIN-II ; ADHESION ; SUPERVILLIN ; KINASE ; QUANTIFICATION ; ADHESOME ; PAXILLIN ; COFILIN ; BINDING |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000381848000001 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374375 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Yingchun |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, 1 West Beichen Rd, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Yajun,Wang, Jinlong,Zhang, Yuanya,et al. Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells[J]. Scientific reports,2016,6:10. |
| APA | Xie, Yajun.,Wang, Jinlong.,Zhang, Yuanya.,Liu, Xiaofei.,Wang, Xiaorong.,...&Wang, Yingchun.(2016).Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells.Scientific reports,6,10. |
| MLA | Xie, Yajun,et al."Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells".Scientific reports 6(2016):10. |
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来源：中国科学院大学
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