Diversity, biogeography, and biodegradation potential of actinobacteria in the deep-sea sediments along the southwest indian ridge
文献类型：期刊论文
| 作者 | Chen, Ping1,2; Zhang, Limin1; Guo, Xiaoxuan1,2; Dai, Xin1,2; Liu, Li3; Xi, Lijun1,6,7; Wang, Jian1; Song, Lei4; Wang, Yuezhu5; Zhu, Yaxin1 |
| 刊名 | Frontiers in microbiology
 |
| 出版日期 | 2016-08-29 |
| 卷号 | 7页码:17 |
| ISSN号 | 1664-302X |
| 关键词 | Community structure Cultivation Biodegradation Marine actinobacteria Microbial diversity Pyrosequencing Southwest indian ridge |
| DOI | 10.3389/fmicb.2016.01340 |
| 通讯作者 | Huang, ying(huangy@im.ac.cn) |
| 英文摘要 | The phylum actinobacteria has been reported to be common or even abundant in deep marine sediments, however, knowledge about the diversity, distribution, and function of actinobacteria is limited. in this study, actinobacterial diversity in the deep sea along the southwest indian ridge (swir) was investigated using both 16s rrna gene pyrosequencing and culture-based methods. the samples were collected at depths of 1662-4000 m below water surface. actinobacterial sequences represented 1.2-9.1% of all microbial 16s rrna gene amplicon sequences in each sample. a total of 5 actinobacterial classes, 17 orders, 28 families, and 52 genera were detected by pyrosequencing, dominated by the classes acidimicrobiia and actinobacteria. differences in actinobacterial community compositions were found among the samples. the community structure showed significant correlations to geochemical factors, notably ph, calcium, total organic carbon, total phosphorus, and total nitrogen, rather than to spatial distance at the scale of the investigation. in addition, 176 strains of the actinobacteria class, belonging to 9 known orders, 18 families, and 29 genera, were isolated. among these cultivated taxa, 8 orders, 13 families, and 15 genera were also recovered by pyrosequencing. at a 97% 16s rrna gene sequence similarity, the pyrosequencing data encompassed 77.3% of the isolates but the isolates represented only 10.3% of the actinobacterial reads. phylogenetic analysis of all the representative actinobacterial sequences and isolates indicated that at least four new orders within the phylum actinobacteria were detected by pyrosequencing. more than half of the isolates spanning 23 genera and all samples demonstrated activity in the degradation of refractory organics, including polycyclic aromatic hydrocarbons and polysaccharides, suggesting their potential ecological functions and biotechnological applications for carbon recycling. |
| WOS关键词 | POLYCYCLIC AROMATIC-HYDROCARBONS ; HIGH-MOLECULAR-WEIGHT ; MARINE-SEDIMENTS ; BACTERIAL DIVERSITY ; MAXIMUM-LIKELIHOOD ; PACIFIC-OCEAN ; MICROBIAL BIOGEOGRAPHY ; COMMUNITY STRUCTURE ; DEGRADING BACTERIA ; HYDROTHERMAL VENTS |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000381990400001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374410 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, Ying |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Informat Network Ctr, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Microbiol, China Gen Microbiol Culture Collect Ctr, Beijing, Peoples R China 5.Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Dis & Hlth Genom, Shanghai, Peoples R China 6.China Univ Petr, State Key Lab Heavy Oil Proc, Qingdao, Peoples R China 7.China Univ Petr, Ctr Bioengn & Biotechnol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Ping,Zhang, Limin,Guo, Xiaoxuan,et al. Diversity, biogeography, and biodegradation potential of actinobacteria in the deep-sea sediments along the southwest indian ridge[J]. Frontiers in microbiology,2016,7:17. |
| APA | Chen, Ping.,Zhang, Limin.,Guo, Xiaoxuan.,Dai, Xin.,Liu, Li.,...&Huang, Ying.(2016).Diversity, biogeography, and biodegradation potential of actinobacteria in the deep-sea sediments along the southwest indian ridge.Frontiers in microbiology,7,17. |
| MLA | Chen, Ping,et al."Diversity, biogeography, and biodegradation potential of actinobacteria in the deep-sea sediments along the southwest indian ridge".Frontiers in microbiology 7(2016):17. |
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来源：中国科学院大学
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