Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy
文献类型：期刊论文
| 作者 | Liu, Zheyi1,2; Wang, Fangjun1; Chen, Jin1,2; Zhou, Ye1,2; Zou, Hanfa1 |
| 刊名 | Journal of chromatography a
 |
| 出版日期 | 2016-08-26 |
| 卷号 | 1461页码:35-41 |
| 关键词 | Multi-phosphopeptide enrichment Competitive substitution Pre-coordination Mass spectrometry Phosphoproteomics |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2016.07.042 |
| 通讯作者 | Wang, fangjun(wangfj@dicp.ac.cn) |
| 英文摘要 | Although many affinity adsorbents have been developed for phosphopeptides enrichment, high specifically capturing the multi-phosphopeptides is still a big challenge. here, we investigated the mechanism of phosphate ion coordination and substitution on affinity adsorbents surfaces and modulated the selectivity of affinity adsorbents to multi-phosphopeptides based on the different capability of mono and multi-phosphopeptides in competitively substituting the pre-coordinated phosphate ions at strong acidic condition. we demonstrated both the species of pre-coordinated phosphate ions and the substituting conditions played crucial roles in modulating the enrichment selectivity to multi-phosphopeptides, and the pre-coordinated affinity materials with relative more surfaces positive charges exhibited better enrichment efficiency due to the cooperative effect of electrostatic interaction and competitive substitution. finally, an enrichment selectivity of 85% to multi-phosphopeptides was feasibly achieved with 66% improvement in identification numbers for complex protein sample extracted from hepg2 cells. data are available via proteomexchange with identifier pxd004252. (c) 2016 elsevier b.v. all rights reserved. |
| WOS关键词 | PHOSPHORYLATED PEPTIDES ; PROTEIN-PHOSPHORYLATION ; MASS-SPECTROMETRY ; TITANIUM-DIOXIDE ; ENRICHMENT ; PHOSPHOPROTEOME ; CHROMATOGRAPHY ; SEPARATION ; NETWORKS ; TIO2 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000381840200005 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374412 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Fangjun |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zheyi,Wang, Fangjun,Chen, Jin,et al. Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy[J]. Journal of chromatography a,2016,1461:35-41. |
| APA | Liu, Zheyi,Wang, Fangjun,Chen, Jin,Zhou, Ye,&Zou, Hanfa.(2016).Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy.Journal of chromatography a,1461,35-41. |
| MLA | Liu, Zheyi,et al."Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy".Journal of chromatography a 1461(2016):35-41. |
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来源：中国科学院大学
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