Grafting voltage and pharmacological sensitivity in potassium channels
文献类型：期刊论文
| 作者 | Lan, Xi1,3; Fan, Chunyan2,3; Ji, Wei2,3; Tian, Fuyun1,3; Xu, Tao2,3; Gao, Zhaobing1,3 |
| 刊名 | Cell research
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 26期号:8页码:935-945 |
| 关键词 | Voltage-gated channels K2p channels Voltage-sensing domain Graft |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2016.57 |
| 通讯作者 | Xu, tao(xutao@ibp.ac.cn) ; Gao, zhaobing(zbgao@simm.ac.cn) |
| 英文摘要 | A classical voltage-gated ion channel consists of four voltage-sensing domains (vsds). however, the roles of each vsd in the channels remain elusive. we developed a gvtdt (graft vsd to dimeric task3 channels that lack endogenous vsds) strategy to produce voltage-gated channels with a reduced number of vsds. task3 channels exhibit a high host tolerance to vsds of various voltage-gated ion channels without interfering with the intrinsic properties of the task3 selectivity filter. the constructed channels, exemplified by the channels grafted with one or two vsds from kv7.1 channels, exhibit classical voltage sensitivity, including voltage-dependent opening and closing. furthermore, the grafted kv7.1 vsd transfers the potentiation activity of benzbromarone, an activator that acts on the vsds of the donor channels, to the constructed channels. our study indicates that one vsd is sufficient to voltage-dependently gate the pore and provides new insight into the roles of vsds. |
| WOS关键词 | DEPENDENT K+ CHANNEL ; GATED PROTON CHANNEL ; C-TYPE INACTIVATION ; GATING CHARGE ; 2-PORE DOMAIN ; ION CHANNELS ; PORE DOMAIN ; EXTRACELLULAR HISTIDINE ; CRYSTAL-STRUCTURE ; SENSING RESIDUES |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000380923900010 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374482 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xu, Tao; Gao, Zhaobing |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, 5 Datun Rd, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lan, Xi,Fan, Chunyan,Ji, Wei,et al. Grafting voltage and pharmacological sensitivity in potassium channels[J]. Cell research,2016,26(8):935-945. |
| APA | Lan, Xi,Fan, Chunyan,Ji, Wei,Tian, Fuyun,Xu, Tao,&Gao, Zhaobing.(2016).Grafting voltage and pharmacological sensitivity in potassium channels.Cell research,26(8),935-945. |
| MLA | Lan, Xi,et al."Grafting voltage and pharmacological sensitivity in potassium channels".Cell research 26.8(2016):935-945. |
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来源：中国科学院大学
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