Molecular dynamics of de novo telomere heterochromatin formation in budding yeast
文献类型：期刊论文
| 作者 | Duan, Yi-Min1; Zhou, Bo-O.1; Peng, Jing1; Tong, Xia-Jing1; Zhang, Qiong-Di1; Zhou, Jin-Qiu1,2 |
| 刊名 | Journal of genetics and genomics
 |
| 出版日期 | 2016-07-20 |
| 卷号 | 43期号:7页码:451-465 |
| 关键词 | Telomere Heterochromatin Nucleosome positioning Sir Yku |
| ISSN号 | 1673-8527 |
| DOI | 10.1016/j.jgg.2016.03.009 |
| 通讯作者 | Zhou, jin-qiu(jqzhou@sibcb.ac.cn) |
| 英文摘要 | In the budding yeast saccharomyces cerevisiae, heterochromatin structure is found at three chromosome regions, which are homothallic mating-type loci, rdna regions and telomeres. to address how telomere heterochromatin is assembled under physiological conditions, we employed a de novo telomere addition system, and analyzed the dynamic chromatin changes of the trp1 reporter gene during telomere elongation. we found that integrating a 255-bp, but not an 81-bp telomeric sequence near the trp1 promoter could trigger sir2 recruitment, active chromatin mark(s)' removal, chromatin compaction and trp1 gene silencing, indicating that the length of the telomeric sequence inserted in the internal region of a chromosome is critical for determining the chromatin state at the proximal region. interestingly, rif1 but not rif2 or yku is indispensable for the formation of intra-chromosomal silent chromatin initiated by telomeric sequence. when an internal short telomeric sequence (e.g., 81 bp) gets exposed to become a de novo telomere, the herterochromatin features, such as sir recruitment, active chromatin mark(s)' removal and chromatin compaction, are detected within a few hours before the de novo telomere reaches a stable length. our results recapitulate the molecular dynamics and reveal a coherent picture of telomere heterochromatin formation. copyright (c) 2016, institute of genetics and developmental biology, chinese academy of sciences, and genetics society of china. published by elsevier limited and science press. all rights reserved. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE TELOMERES ; NUCLEOSOME CORE PARTICLE ; DNA-BINDING PROTEIN ; OF-FUNCTION MUTANTS ; SILENT CHROMATIN ; HISTONE H3 ; ANGSTROM RESOLUTION ; SIR2/SIR4 COMPLEX ; CRYSTAL-STRUCTURE ; GENE-EXPRESSION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS类目 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000381009700004 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374492 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhou, Jin-Qiu |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol,Shanghai Inst Biochem & Ce, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.Shanghai Tech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Yi-Min,Zhou, Bo-O.,Peng, Jing,et al. Molecular dynamics of de novo telomere heterochromatin formation in budding yeast[J]. Journal of genetics and genomics,2016,43(7):451-465. |
| APA | Duan, Yi-Min,Zhou, Bo-O.,Peng, Jing,Tong, Xia-Jing,Zhang, Qiong-Di,&Zhou, Jin-Qiu.(2016).Molecular dynamics of de novo telomere heterochromatin formation in budding yeast.Journal of genetics and genomics,43(7),451-465. |
| MLA | Duan, Yi-Min,et al."Molecular dynamics of de novo telomere heterochromatin formation in budding yeast".Journal of genetics and genomics 43.7(2016):451-465. |
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来源：中国科学院大学
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