Development of a synthetic oxytetracycline-inducible expression system for streptomycetes using de novo characterized genetic parts
文献类型：期刊论文
| 作者 | Wang, Weishan1; Yang, Tongjian1,2; Li, Yihong1; Li, Shanshan1; Yin, Shouliang1; Styles, Kathryn3,4; Corre, Christophe3,4; Yang, Keqian1 |
| 刊名 | Acs synthetic biology
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 5期号:7页码:765-773 |
| 关键词 | Inducible expression system Streptomycetes Genetic parts Wide-range strength Biosynthetic gene cluster Natural product |
| ISSN号 | 2161-5063 |
| DOI | 10.1021/acssynbio.6b00087 |
| 通讯作者 | Yang, keqian(yangkq@im.ac.cn) |
| 英文摘要 | Precise control of gene expression using exogenous factors is of great significance. to develop ideal inducible expression systems for streptomycetes, new genetic parts, oxytetracycline responsive repressor otrr, operator otro, and promoter otrbp from streptomyces rimosus, were selected de novo and characterized in vivo and in vitro. otrr showed strong affinity to otro (k-d = 1.7 x 10(-10) m) and oxytetracycline induced dissociation of the otrr/dna complex in a concentration-dependent manner. on the basis of these genetic parts, a synthetic inducible expression system potr* was optimized. induction of potr* with 0.01-4 mu m of oxytetracycline triggered a wide-range expression level of gfp reporter gene in different streptomyces species. benchrnarking potr* against the widely used constitutive promoters erme* and kasop* revealed greatly enhanced levels of expression when potr* was fully induced. finally, potr* was used as a tool to activate and optimize the expression of the silent jadomycin biosynthetic gene cluster in streptomyces venezuelae. altogether, the synthetic port* presents a new versatile tool for fine-tuning gene expression in streptomycetes. |
| WOS关键词 | TRANSCRIPTIONAL REGULATORS ; CONSTITUTIVE PROMOTERS ; TETR FAMILY ; IDENTIFICATION ; DNA ; BIOSYNTHESIS ; BACTERIA ; BIOLOGY ; DESIGN ; CONSTRUCTION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods |
| 语种 | 英语 |
| WOS记录号 | WOS:000380183000025 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374521 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, Keqian |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, 1 West Beichen Rd, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England 4.Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England |
| 推荐引用方式 GB/T 7714 | Wang, Weishan,Yang, Tongjian,Li, Yihong,et al. Development of a synthetic oxytetracycline-inducible expression system for streptomycetes using de novo characterized genetic parts[J]. Acs synthetic biology,2016,5(7):765-773. |
| APA | Wang, Weishan.,Yang, Tongjian.,Li, Yihong.,Li, Shanshan.,Yin, Shouliang.,...&Yang, Keqian.(2016).Development of a synthetic oxytetracycline-inducible expression system for streptomycetes using de novo characterized genetic parts.Acs synthetic biology,5(7),765-773. |
| MLA | Wang, Weishan,et al."Development of a synthetic oxytetracycline-inducible expression system for streptomycetes using de novo characterized genetic parts".Acs synthetic biology 5.7(2016):765-773. |
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来源：中国科学院大学
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