A glutamic acid decarboxylase (cggad) highly expressed in hemocytes of pacific oyster crassostrea gigas
文献类型：期刊论文
| 作者 | Li, Meijia1,3; Wang, Lingling2; Qiu, Limei1; Wang, Weilin1,3; Xin, Lusheng1,3; Xu, Jiachao1,3; Wang, Hao1; Song, Linsheng2 |
| 刊名 | Developmental and comparative immunology
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 63页码:56-65 |
| 关键词 | Crassostrea gigas Gaba synthase Immune response Hemocyte specific |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2016.05.010 |
| 通讯作者 | Wang, lingling(wanglingling@ms.qdio.ac.cn) ; Song, linsheng() |
| 英文摘要 | Glutamic acid decarboxylase (gad), a rate-limiting enzyme to catalyze the reaction converting the excitatory neurotransmitter glutamate to inhibitory neurotransmitter gamma-aminobutyric acid (gaba), not only functions in nervous system, but also plays important roles in immunomodulation in vertebrates. however, gad has rarely been reported in invertebrates, and never in molluscs. in the present study, one gad homologue (designed as cggad) was identified from pacific oyster crassostrea gigas. the full length cdna of cggad was 1689 bp encoding a polypeptide of 562 amino acids containing a conserved pyridoxal-dependent decarboxylase domain. cggad mrna and protein could be detected in ganglion and hemocytes of oysters, and their abundance in hemocytes was unexpectedly much higher than those in ganglion. more importantly, cggad was mostly located in those granulocytes without phagocytic capacity in oysters, and could dynamically respond to lps stimulation. further, after being transfected into hek293 cells, cggad could promote the production of gaba. collectively, these findings suggested that cggad, as a gaba synthase and molecular marker of gabaergic system, was mainly distributed in hemocytes and ganglion and involved in neuroendocrine-immune regulation network in oysters, which also provided a novel insight to the co-evolution between nervous system and immune system. (c) 2016 elsevier ltd. all rights reserved. |
| WOS关键词 | GABAERGIC NERVOUS-SYSTEM ; CAENORHABDITIS-ELEGANS ; CHLAMYS-FARRERI ; INHIBITORY ROLE ; GABA ; IMMUNE ; DISEASE ; IMMUNOMODULATION ; INVERTEBRATES ; HETEROGENEITY |
| WOS研究方向 | Immunology ; Zoology |
| WOS类目 | Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000380623300007 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374524 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Lingling; Song, Linsheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Meijia,Wang, Lingling,Qiu, Limei,et al. A glutamic acid decarboxylase (cggad) highly expressed in hemocytes of pacific oyster crassostrea gigas[J]. Developmental and comparative immunology,2016,63:56-65. |
| APA | Li, Meijia.,Wang, Lingling.,Qiu, Limei.,Wang, Weilin.,Xin, Lusheng.,...&Song, Linsheng.(2016).A glutamic acid decarboxylase (cggad) highly expressed in hemocytes of pacific oyster crassostrea gigas.Developmental and comparative immunology,63,56-65. |
| MLA | Li, Meijia,et al."A glutamic acid decarboxylase (cggad) highly expressed in hemocytes of pacific oyster crassostrea gigas".Developmental and comparative immunology 63(2016):56-65. |
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来源：中国科学院大学
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