Molecular cloning and sexually dimorphic expression of wnt4 in olive flounder (paralichthys olivaceus)
文献类型：期刊论文
| 作者 | Weng, Shenda1,2; You, Feng1,3; Fan, Zhaofei1,2; Wang, Lijuan1,2; Wu, Zhihao1,3; Zou, Yuxia1,3 |
| 刊名 | Fish physiology and biochemistry
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 42期号:4页码:1167-1176 |
| ISSN号 | 0920-1742 |
| 关键词 | Olive flounder Wnt4 Molecular cloning Sexually dimorphic expression Gonadal differentiation Gonadal development |
| DOI | 10.1007/s10695-016-0206-6 |
| 通讯作者 | You, feng(youfeng@qdio.ac.cn) ; Zou, yuxia(zouyuxia@qdio.ac.cn) |
| 英文摘要 | Wnt4 (wingless-type mmtv integration site family, member 4) is regarded as a key regulator of gonad differentiation in mammalians. however, the potential role of wnt4 in teleosts during gonad differentiation and development is still unclear. the full-length cdna sequence of wnt4 in olive flounder (paralichthys olivaceus) was obtained using race (rapid amplification of cdna ends) technique. the wnt4 orf contains 1059 nucleotides, encoding a protein with a signal peptide domain and a wnt family domain. expression in tissues of adult flounders was analyzed by real-time rt-pcr. the results showed that wnt4 was widely expressed in multiple tissues of flounders, and the expression level was significantly higher in ovary than in testis. then wnt4 expression pattern was investigated during gonadal differentiation period and at gonadal development stages (i-v). the results showed the expression levels were significantly higher in testis than in ovary during gonadal differentiation. notably, wnt4 expression had a very significant increase before testis differentiation. at gonad different developmental stages, there was no expression signal at stage i or stage ii, and the expression of wnt4 was much stronger in ovary than in testis at stage iii and stage iv, followed by a faint expression in stage v in both sexes. our results imply that cloned wnt4 could be wnt4a. it is a sex-related gene and its expression pattern in gonadal differentiation period of flounder is different from that in mammalians or other teleosts. flounder wnt4 might play more important role in testis than in ovary during gonadal differentiation. |
| WOS关键词 | GONADAL SEX-DIFFERENTIATION ; JAPANESE FLOUNDER ; GENE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
| WOS类目 | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000379734500009 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374548 |
| 专题 | 中国科学院大学 |
| 通讯作者 | You, Feng; Zou, Yuxia |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Weng, Shenda,You, Feng,Fan, Zhaofei,et al. Molecular cloning and sexually dimorphic expression of wnt4 in olive flounder (paralichthys olivaceus)[J]. Fish physiology and biochemistry,2016,42(4):1167-1176. |
| APA | Weng, Shenda,You, Feng,Fan, Zhaofei,Wang, Lijuan,Wu, Zhihao,&Zou, Yuxia.(2016).Molecular cloning and sexually dimorphic expression of wnt4 in olive flounder (paralichthys olivaceus).Fish physiology and biochemistry,42(4),1167-1176. |
| MLA | Weng, Shenda,et al."Molecular cloning and sexually dimorphic expression of wnt4 in olive flounder (paralichthys olivaceus)".Fish physiology and biochemistry 42.4(2016):1167-1176. |
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来源：中国科学院大学
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