Extending the spatiotemporal resolution of super-resolution microscopies using photomodulatable fluorescent proteins
文献类型：期刊论文
| 作者 | Zhang, Mingshu1,2; Fu, Zhifei1,2,3; Xu, Pingyong1,2 |
| 刊名 | Journal of innovative optical health sciences
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 9期号:3页码:11 |
| 关键词 | Super-resolution Pmfp Mgeos Meos3 Skylan-s Skylan-ns |
| ISSN号 | 1793-5458 |
| DOI | 10.1142/s1793545816300093 |
| 通讯作者 | Xu, pingyong(pyxu@ibp.ac.cn) |
| 英文摘要 | In the past two decades, various super-resolution (sr) microscopy techniques have been developed to break the diffrraction limit using subdiffrraction excitation to spatially modulate the fluorescence emission. photomodulatable fluorescent proteins (fps) can be activated by light of specific wavelengths to produce either stochastic or patterned subdiffrraction excitation, resulting in improved optical resolution. in this review, we focus on the recently developed photomodulatable fps or commonly used sr microscopies and discuss the concepts and strategies for optimizing and selecting the biochemical and photophysical properties of pmfps to improve the spatiotemporal resolution of sr techniques, especially time-lapse live-cell sr techniques. |
| WOS关键词 | STRUCTURED-ILLUMINATION MICROSCOPY ; SINGLE-MOLECULE LOCALIZATION ; FLUCTUATION IMAGING SOFI ; DIFFRACTION BARRIER ; BREAKING ; LIMIT ; TRACKING ; BRIGHT ; NANOSCOPY ; PROBES |
| WOS研究方向 | Optics ; Radiology, Nuclear Medicine & Medical Imaging |
| WOS类目 | Optics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000379752700005 |
| 出版者 | WORLD SCIENTIFIC PUBL CO PTE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374554 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xu, Pingyong |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Key Lab RNA Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Beijing Key Lab Noncoding RNA, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Mingshu,Fu, Zhifei,Xu, Pingyong. Extending the spatiotemporal resolution of super-resolution microscopies using photomodulatable fluorescent proteins[J]. Journal of innovative optical health sciences,2016,9(3):11. |
| APA | Zhang, Mingshu,Fu, Zhifei,&Xu, Pingyong.(2016).Extending the spatiotemporal resolution of super-resolution microscopies using photomodulatable fluorescent proteins.Journal of innovative optical health sciences,9(3),11. |
| MLA | Zhang, Mingshu,et al."Extending the spatiotemporal resolution of super-resolution microscopies using photomodulatable fluorescent proteins".Journal of innovative optical health sciences 9.3(2016):11. |
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来源：中国科学院大学
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