Enhanced dstorm imaging using fluorophores interacting with cucurbituril
文献类型：期刊论文
| 作者 | Zhang, Min1,2; Gao, Jing1,2; Chen, Junling1,2; Cai, Mingjun1; Jiang, Junguang1; Tian, Zhiyuan3; Wang, Hongda1 |
| 刊名 | Science china-chemistry
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 59期号:7页码:848-852 |
| 关键词 | Super-resolution imaging Cucurbituril Photophysical Localization precision |
| ISSN号 | 1674-7291 |
| DOI | 10.1007/s11426-015-0535-0 |
| 通讯作者 | Tian, zhiyuan(zytian@ucas.ac.cn) ; Wang, hongda(hdwang@ciac.ac.cn) |
| 英文摘要 | Advanced fluorescence microscopy including single-molecule localization-based super-resolution imaging techniques requires bright and photostable dyes or proteins as fluorophores. the photophysical properties of fluorophores have been proven to be crucial for super-resolution microscopy's localization precision and imaging resolution. fluorophores tamra and atto rho6g, which can interact with macrocyclic host cucurbit[7]uril (cb7) to form host-guest compounds, were found to improve the fluorescence intensity and lifetimes of these dyes. we enhanced the localization precision of direct stochastic optical reconstruction microscopy (dstorm) by introducing cb7 into the imaging buffer, and showed that the number of photons as well as localizations of both tamra and atto rho6g increase over 2 times. |
| WOS关键词 | SUPERRESOLUTION FLUORESCENCE MICROSCOPY ; COMPLEXES ; DYES |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000379509500011 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374555 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tian, Zhiyuan; Wang, Hongda |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Gao, Jing,Chen, Junling,et al. Enhanced dstorm imaging using fluorophores interacting with cucurbituril[J]. Science china-chemistry,2016,59(7):848-852. |
| APA | Zhang, Min.,Gao, Jing.,Chen, Junling.,Cai, Mingjun.,Jiang, Junguang.,...&Wang, Hongda.(2016).Enhanced dstorm imaging using fluorophores interacting with cucurbituril.Science china-chemistry,59(7),848-852. |
| MLA | Zhang, Min,et al."Enhanced dstorm imaging using fluorophores interacting with cucurbituril".Science china-chemistry 59.7(2016):848-852. |
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来源：中国科学院大学
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