Human bocavirus ns1 and ns1-70 proteins inhibit tnf-alpha-mediated activation of nf-kappa b by targeting p65
文献类型：期刊论文
| 作者 | Liu, Qingshi1,2; Zhang, Zhenfeng1; Zheng, Zhenhua1; Zheng, Caishang1; Liu, Yan1; Hu, Qinxue3; Ke, Xianliang1; Wang, Hanzhong1 |
| 刊名 | Scientific reports
 |
| 出版日期 | 2016-06-22 |
| 卷号 | 6页码:12 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep28481 |
| 通讯作者 | Wang, hanzhong(wanghz@wh.iov.cn) |
| 英文摘要 | Human bocavirus (hbov), a parvovirus, is a single-stranded dna etiologic agent causing lower respiratory tract infections in young children worldwide. nuclear factor kappa b (nf-kappa b) transcription factors play crucial roles in clearance of invading viruses through activation of many physiological processes. previous investigation showed that hbov infection could significantly upregulate the level of tnf-alpha which is a strong nf-kappa b stimulator. here we investigated whether hbov proteins modulate tnf-alpha-mediated activation of the nf-kappa b signaling pathway. we showed that hbov ns1 and ns1-70 proteins blocked nf-kappa b activation in response to tnf-alpha. overexpression of tnf receptor-associated factor 2 (traf2)-, i kappa b kinase alpha (ikk alpha)-, i kappa b kinase beta (ikk beta)-, constitutively active mutant of ikk beta (ikk beta ss/ee)-, or p65-induced nf-kappa b activation was inhibited by ns1 and ns1-70. furthermore, ns1 and ns1-70 didn't interfere with tnf-alpha-mediated i kappa b alpha phosphorylation and degradation, nor p65 nuclear translocation. coimmunoprecipitation assays confirmed the interaction of both ns1 and ns1-70 with p65. of note, ns1 but not ns1-70 inhibited tnf-alpha-mediated p65 phosphorylation at ser536. our findings together indicate that hbov ns1 and ns1-70 inhibit nf-kappa b activation. this is the first time that hbov has been shown to inhibit nf-kappa b activation, revealing a potential immune-evasion mechanism that is likely important for hbov pathogenesis. |
| WOS关键词 | RESPIRATORY-TRACT INFECTION ; POSTTRANSLATIONAL MODIFICATIONS ; EPITHELIAL-CELLS ; IMMUNE-RESPONSE ; GENE-EXPRESSION ; CHILDREN ; PATHWAY ; VIRUSES ; PHOSPHORYLATION ; UBIQUITINATION |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000380000700001 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374573 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Hanzhong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Qingshi,Zhang, Zhenfeng,Zheng, Zhenhua,et al. Human bocavirus ns1 and ns1-70 proteins inhibit tnf-alpha-mediated activation of nf-kappa b by targeting p65[J]. Scientific reports,2016,6:12. |
| APA | Liu, Qingshi.,Zhang, Zhenfeng.,Zheng, Zhenhua.,Zheng, Caishang.,Liu, Yan.,...&Wang, Hanzhong.(2016).Human bocavirus ns1 and ns1-70 proteins inhibit tnf-alpha-mediated activation of nf-kappa b by targeting p65.Scientific reports,6,12. |
| MLA | Liu, Qingshi,et al."Human bocavirus ns1 and ns1-70 proteins inhibit tnf-alpha-mediated activation of nf-kappa b by targeting p65".Scientific reports 6(2016):12. |
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来源：中国科学院大学
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