Direct sensing and signal transduction during bacterial chemotaxis toward aromatic compounds in comamonas testosteroni
文献类型：期刊论文
| 作者 | Huang, Zhou1,2,3; Ni, Bin1,2; Jiang, Cheng-Ying1,2,4; Wu, Yu-Fan1,2,3; He, Yun-Zhe1,2,3; Parales, Rebecca E.5; Liu, Shuang-Jiang1,2,3,4 |
| 刊名 | Molecular microbiology
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 101期号:2页码:224-237 |
| ISSN号 | 0950-382X |
| DOI | 10.1111/mmi.13385 |
| 通讯作者 | Jiang, cheng-ying(jiangcy@im.ac.cn) ; Liu, shuang-jiang(liusj@im.ac.cn) |
| 英文摘要 | Micro-organisms sense and chemotactically respond to aromatic compounds. although the existence of chemoreceptors that bind to aromatic attractants and subsequently trigger chemotaxis have long been speculated, such a chemoreceptor has not been demonstrated. in this report, we demonstrated that the chemoreceptor mcp2901 from comamonas testosteroni cnb-1 binds to aromatic compounds and initiates downstream chemotactic signaling in addition to its ability to trigger chemotaxis via citrate binding. the function of gene mcp2901 was investigated by genetic deletion from cnb-1 and genetic complementation of the methyl-accepting chemotaxis protein (mcp)-null mutant cnb-120. results showed that the expression of mcp2901 in the mcp-null mutant restored chemotaxis toward nine tested aromatic compounds and nine carboxylic acids. isothermal titration calorimetry (itc) analyses demonstrated that the ligand-binding domain of mcp2901 (mcp2901lbd) bound to citrate, and weakly to gentisate and 4-hydroxybenzoate. additionally, itc assays indicated that mcp2901lbd bound strongly to 2,6-dihydroxybenzoate and 2-hydroxybenzoate, which are isomers of gentisate and 4-hydroxybenzoate respectively that are not metabolized by cnb-1. agarose-in-plug and capillary assays showed that these two molecules serve as chemoattractants for cnb-1. through constructing membrane-like mcp2901-inserted nanodiscs and phosphorelay activity assays, we demonstrated that 2,6-dihydroxybenzoate and 2-hydroxybenzoate altered kinase activity of chea. this is the first evidence of an mcp binding to an aromatic molecule and triggering signal transduction for bacterial chemotaxis. |
| WOS关键词 | PSEUDOMONAS-PUTIDA F1 ; ESCHERICHIA-COLI ; STRAIN CNB-1 ; CHEMORECEPTOR ; IDENTIFICATION ; ACID ; POLLUTANTS ; PROTEINS ; TAXIS ; METABOLISM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000379968900005 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374587 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jiang, Cheng-Ying; Liu, Shuang-Jiang |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Environm Microbiol Res Ctr, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.IMCAS RCEECAS Joint Lab Environm Microbial Techno, Beijing, Peoples R China 5.Univ Calif Davis, Dept Microbiol & Mol Genet, Davis, CA 95616 USA |
| 推荐引用方式 GB/T 7714 | Huang, Zhou,Ni, Bin,Jiang, Cheng-Ying,et al. Direct sensing and signal transduction during bacterial chemotaxis toward aromatic compounds in comamonas testosteroni[J]. Molecular microbiology,2016,101(2):224-237. |
| APA | Huang, Zhou.,Ni, Bin.,Jiang, Cheng-Ying.,Wu, Yu-Fan.,He, Yun-Zhe.,...&Liu, Shuang-Jiang.(2016).Direct sensing and signal transduction during bacterial chemotaxis toward aromatic compounds in comamonas testosteroni.Molecular microbiology,101(2),224-237. |
| MLA | Huang, Zhou,et al."Direct sensing and signal transduction during bacterial chemotaxis toward aromatic compounds in comamonas testosteroni".Molecular microbiology 101.2(2016):224-237. |
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来源：中国科学院大学
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