Genistein sensitizes sarcoma cells in vitro and in vivo by enhancing apoptosis and by inhibiting dsb repair pathways
文献类型：期刊论文
| 作者 | Liu, X. X.1,2,3; Sun, C.1,2,3; Jin, X. D.1,2,3; Li, P.1,2,3; Zheng, X. G.1,2,3,4; Zhao, T.1,2,3; Li, Q.1,2,3 |
| 刊名 | Journal of radiation research
 |
| 出版日期 | 2016-06-01 |
| 卷号 | 57期号:3页码:227-237 |
| 关键词 | Sarcoma Radiosensitivity Genistein Dna damage repair Apoptosis |
| ISSN号 | 0449-3060 |
| DOI | 10.1093/jrr/rrv091 |
| 通讯作者 | Li, q.(liqiang@impcas.ac.cn) |
| 英文摘要 | The aim of this work was to investigate the radiosensitization effects of genistein on mice sarcoma cells and the corresponding biological mechanisms in vitro and in vivo. using the non-toxic dosage of 10 mu m genistein, the sensitizer enhancement ratios after exposure to x-rays at 50% cell survival (ic50) was 1.45 for s180 cells. for mice cotreated with genistein and x-rays, the excised tumor tissues had reduced blood vessels and decreased size and volume compared with the control and irradiation-only groups. moreover, a significant increase in apoptosis was accompanied by upregulation of bax and downregulation of bcl-2 in the mitochondria, and lots of cytochrome c being transferred to the cytoplasm. furthermore, x-rays combined with genistein inhibited the activity of dna-pkcs, so dna-injured sites were dominated by ku70/80, leading to incompleteness of homologous recombination (hr) and non-homologous end-joining (nhej) repairs and the eventual occurrence of cell apoptosis. our study, for the first time, demonstrated that genistein sensitized sarcoma cells to x-rays and that this radiosensitizing effect depended on induction of the mitochondrial apoptosis pathway and inhibition of the double-strand break (dsb) repair pathways. |
| WOS关键词 | DEPENDENT PROTEIN-KINASE ; BREAST-CANCER CELLS ; PROSTATE-CANCER ; CATALYTIC SUBUNIT ; HOMOLOGOUS RECOMBINATION ; MAMMALIAN-CELLS ; DNA-REPAIR ; RADIOSENSITIVITY ; RADIATION ; OSTEOSARCOMA |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging |
| WOS类目 | Biology ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000378758100004 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374614 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Q. |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 3.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Gansu, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, X. X.,Sun, C.,Jin, X. D.,et al. Genistein sensitizes sarcoma cells in vitro and in vivo by enhancing apoptosis and by inhibiting dsb repair pathways[J]. Journal of radiation research,2016,57(3):227-237. |
| APA | Liu, X. X..,Sun, C..,Jin, X. D..,Li, P..,Zheng, X. G..,...&Li, Q..(2016).Genistein sensitizes sarcoma cells in vitro and in vivo by enhancing apoptosis and by inhibiting dsb repair pathways.Journal of radiation research,57(3),227-237. |
| MLA | Liu, X. X.,et al."Genistein sensitizes sarcoma cells in vitro and in vivo by enhancing apoptosis and by inhibiting dsb repair pathways".Journal of radiation research 57.3(2016):227-237. |
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来源：中国科学院大学
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