中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Diammonium phosphate stimulates transcription of l-lactate dehydrogenase leading to increased l-lactate production in the thermotolerant bacillus coagulans strain

文献类型:期刊论文

作者Sun, Lifan1,2; Li, Yanfeng1,3; Wang, Limin1; Wang, Yanping2; Yu, Bo1
刊名Applied microbiology and biotechnology
出版日期2016-08-01
卷号100期号:15页码:6653-6660
关键词L-lactate Bacillus coagulans Diammonium phosphate L-lactate dehydrogenase Thermotolerant
ISSN号0175-7598
DOI10.1007/s00253-016-7379-x
通讯作者Yu, bo(yub@im.ac.cn)
英文摘要Exploration of cost-effective fermentation substrates for efficient lactate production is an important economic objective. although some organic nitrogen sources are also cheaper, inorganic nitrogen salts for lactate fermentation have additional advantages in facilitating downstream procedures and significantly improving the commercial competitiveness of lactate production. in this study, we first established an application of diammonium phosphate to replace yeast extract with a reduced 90 % nitrogen cost for a thermotolerant bacillus coagulans strain. in vivo enzymatic and transcriptional analyses demonstrated that diammonium phosphate stimulates the gene expression of l-lactate dehydrogenase, thus providing higher specific enzyme activity in vivo and increasing l-lactic acid production. this new information provides a foundation for establishing a cost-effective process for polymer-grade l-lactic acid production in an industrial setting.
WOS关键词D-LACTIC ACID ; HIGH-OPTICAL-PURITY ; ESCHERICHIA-COLI W3110 ; FERMENTATIVE PRODUCTION ; LACTOBACILLUS-CASEI ; EFFICIENT PRODUCER ; NITROGEN-SOURCES ; SUPPLEMENTATION ; STARCH
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000379317300011
出版者SPRINGER
URI标识http://www.irgrid.ac.cn/handle/1471x/2374626
专题中国科学院大学
通讯作者Yu, Bo
作者单位1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China
2.Tianjin Univ Sci & Technol, Sch Food Engn & Biol Technol, Tianjin 300457, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Sun, Lifan,Li, Yanfeng,Wang, Limin,et al. Diammonium phosphate stimulates transcription of l-lactate dehydrogenase leading to increased l-lactate production in the thermotolerant bacillus coagulans strain[J]. Applied microbiology and biotechnology,2016,100(15):6653-6660.
APA Sun, Lifan,Li, Yanfeng,Wang, Limin,Wang, Yanping,&Yu, Bo.(2016).Diammonium phosphate stimulates transcription of l-lactate dehydrogenase leading to increased l-lactate production in the thermotolerant bacillus coagulans strain.Applied microbiology and biotechnology,100(15),6653-6660.
MLA Sun, Lifan,et al."Diammonium phosphate stimulates transcription of l-lactate dehydrogenase leading to increased l-lactate production in the thermotolerant bacillus coagulans strain".Applied microbiology and biotechnology 100.15(2016):6653-6660.

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来源:中国科学院大学

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