Diammonium phosphate stimulates transcription of l-lactate dehydrogenase leading to increased l-lactate production in the thermotolerant bacillus coagulans strain
文献类型：期刊论文
| 作者 | Sun, Lifan1,2; Li, Yanfeng1,3; Wang, Limin1; Wang, Yanping2; Yu, Bo1 |
| 刊名 | Applied microbiology and biotechnology
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 100期号:15页码:6653-6660 |
| 关键词 | L-lactate Bacillus coagulans Diammonium phosphate L-lactate dehydrogenase Thermotolerant |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-016-7379-x |
| 通讯作者 | Yu, bo(yub@im.ac.cn) |
| 英文摘要 | Exploration of cost-effective fermentation substrates for efficient lactate production is an important economic objective. although some organic nitrogen sources are also cheaper, inorganic nitrogen salts for lactate fermentation have additional advantages in facilitating downstream procedures and significantly improving the commercial competitiveness of lactate production. in this study, we first established an application of diammonium phosphate to replace yeast extract with a reduced 90 % nitrogen cost for a thermotolerant bacillus coagulans strain. in vivo enzymatic and transcriptional analyses demonstrated that diammonium phosphate stimulates the gene expression of l-lactate dehydrogenase, thus providing higher specific enzyme activity in vivo and increasing l-lactic acid production. this new information provides a foundation for establishing a cost-effective process for polymer-grade l-lactic acid production in an industrial setting. |
| WOS关键词 | D-LACTIC ACID ; HIGH-OPTICAL-PURITY ; ESCHERICHIA-COLI W3110 ; FERMENTATIVE PRODUCTION ; LACTOBACILLUS-CASEI ; EFFICIENT PRODUCER ; NITROGEN-SOURCES ; SUPPLEMENTATION ; STARCH |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000379317300011 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374626 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, Bo |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 2.Tianjin Univ Sci & Technol, Sch Food Engn & Biol Technol, Tianjin 300457, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Lifan,Li, Yanfeng,Wang, Limin,et al. Diammonium phosphate stimulates transcription of l-lactate dehydrogenase leading to increased l-lactate production in the thermotolerant bacillus coagulans strain[J]. Applied microbiology and biotechnology,2016,100(15):6653-6660. |
| APA | Sun, Lifan,Li, Yanfeng,Wang, Limin,Wang, Yanping,&Yu, Bo.(2016).Diammonium phosphate stimulates transcription of l-lactate dehydrogenase leading to increased l-lactate production in the thermotolerant bacillus coagulans strain.Applied microbiology and biotechnology,100(15),6653-6660. |
| MLA | Sun, Lifan,et al."Diammonium phosphate stimulates transcription of l-lactate dehydrogenase leading to increased l-lactate production in the thermotolerant bacillus coagulans strain".Applied microbiology and biotechnology 100.15(2016):6653-6660. |
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来源：中国科学院大学
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