Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry
文献类型：期刊论文
| 作者 | Gu, Qun; Shi, Xianzhe; Yin, Peiyuan; Gao, Peng; Lu, Xin; Xu, Gumang |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2008-02-25 |
| 卷号 | 609期号:2页码:192-200 |
| 关键词 | catecholamine metabolite liquid chromatography tandem mass spectrometry adrenal gland |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | two simple, rapid and specific analytical methods for 13 catecholamines and their metabolites have been developed based on liquid chromatography tandem mass spectrometry in a multiple reaction monitoring mode. tyrosine, dopamine, dihydroxyphenylalanine, epinephrine, norepinephrine, 3-methoxytyramine, normetanephrine, metanephrine and isoproterenol (internal standard) were separated on a kromasil (tm) cyano, analytical column by a mobile phase consisting of 60% (v/v) acetonitrile and 40% (v/v) water adjusted with formic acid to ph 3.0, and detected by positive ionization electrospray tandem mass spectrometry. while vanillymandelic acid, 3,4-dihydroxymandelic acid, homovanillic acid, 3,4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylglycol and 5-hydroxy-2-indolecarboxylic acid (internal standard) were separated on a reversed-phase shim-pak vp-ods column with the mobile phase of 60% (v/v) acetonitrile, and 40% (v/v) water adjusted with formic acid to ph 4.5 and detected in the negative ionization electrospray tandem mass spectrometry. the influence of various parameters such as column type and mobile phase composition on separation and sensitivity were investigated. the limits of detection were in the range of 0.5-20 ng ml(-1). the mean recoveries determined from three different concentrations of each analyte were above 85.4%. the precision of the method calculated as relative standard deviation was lower than 5.3%. deduced from the results of real sample analysis, adrenal gland synthesizes and stores the catecholamine hormones norepinephrine and epinephrine. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | plasma-free metanephrine ; solid-phase extraction ; capillary-electrophoresis ; electrochemical detection ; fluorescence detection ; l-dopa ; neurotransmitters ; epinephrine ; urine ; norepinephrine |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000253616500007 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/100427]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Qun,Shi, Xianzhe,Yin, Peiyuan,et al. Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry[J]. analytica chimica acta,2008,609(2):192-200. |
| APA | Gu, Qun,Shi, Xianzhe,Yin, Peiyuan,Gao, Peng,Lu, Xin,&Xu, Gumang.(2008).Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry.analytica chimica acta,609(2),192-200. |
| MLA | Gu, Qun,et al."Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry".analytica chimica acta 609.2(2008):192-200. |
入库方式： OAI收割
来源：大连化学物理研究所
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