Development of a comprehensive two-dimensional hydrophilic interaction chromatography/quadrupole time-of-flight mass spectrometry system and its application in separation and identification of saponins from Quillaja saponaria
文献类型：期刊论文
| 作者 | Wang, Yuan; Lu, Xin; Xu, Guowang |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2008-02-15 |
| 卷号 | 1181期号:1-2页码:51-59 |
| 关键词 | HILIC comprehensive two-dimensional liquid chromatography orthogonality quillaja saponins |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | hydrophilic interaction chromatography (hilic) was used in two dimensions in a comprehensive two-dimensional hilic hyphenated with a quadrupole time-of-flight mass spectrometry (hilic x hilic-q-tof-ms) system for the analysis of complex samples of hydrophilic compounds. a tskgel amide-80 column was employed as the first dimension, and a short polyhydroxyethyl a column was as the second dimension. the column system showed moderate orthogonality at defined operational conditions. a high speed q-tof-ms detector as a third complementary dimension significantly improved the peak capacity. the separation capability of the developed hilic x hilic-q-tof-ms system was tested by separating an extract from quillaja saponaria. the major components, quillaja saponins, in the extract were well identified by means of [m-h](-) ions, characteristic product ions, and their two-dimensional retention behaviors. several pairs of isomers, which were often co-eluted on conventional lc-ms methods and had similar fragmentation characteristics in ms/ms spectra, were well separated on the two-dimensional system based on their different hydrophilicity. the developed comprehensive two-dimensional hilic system demonstrates unique selectivity for hydrophilic compounds and satisfactory peak capacity and resolution for analogues by making sufficient use of two-dimensional separation plane. (c) 2007 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | phase liquid-chromatography ; peptide separation ; sample preparation ; stationary phases ; online ; hplc ; orthogonality ; column ; triacylglycerols ; selectivity |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000253506600006 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/100429]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yuan,Lu, Xin,Xu, Guowang. Development of a comprehensive two-dimensional hydrophilic interaction chromatography/quadrupole time-of-flight mass spectrometry system and its application in separation and identification of saponins from Quillaja saponaria[J]. journal of chromatography a,2008,1181(1-2):51-59. |
| APA | Wang, Yuan,Lu, Xin,&Xu, Guowang.(2008).Development of a comprehensive two-dimensional hydrophilic interaction chromatography/quadrupole time-of-flight mass spectrometry system and its application in separation and identification of saponins from Quillaja saponaria.journal of chromatography a,1181(1-2),51-59. |
| MLA | Wang, Yuan,et al."Development of a comprehensive two-dimensional hydrophilic interaction chromatography/quadrupole time-of-flight mass spectrometry system and its application in separation and identification of saponins from Quillaja saponaria".journal of chromatography a 1181.1-2(2008):51-59. |
入库方式： OAI收割
来源：大连化学物理研究所
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