Physical interaction and assembly of bacillus subtilis spore coat proteins cote and cotz studied by atomic force microscopy
文献类型：期刊论文
| 作者 | Liu, Huiqing1,2; Qiao, Haiyan3; Krajcikova, Daniela4; Zhang, Zhe1; Wang, Hongda1; Barak, Imrich4; Tang, Jilin1 |
| 刊名 | Journal of structural biology
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 195期号:2页码:245-251 |
| 关键词 | Atomic force microscopy Single molecule force spectroscopy Protein-protein interaction Self-assembly Cote Cotz |
| ISSN号 | 1047-8477 |
| DOI | 10.1016/j.jsb.2016.06.010 |
| 通讯作者 | Tang, jilin(jltang@ciac.ac.cn) |
| 英文摘要 | The spore of bacillus subtilis, a dormant type of cell, is surrounded by a complex multilayered protein structure known as the coat. it is composed of over 70 proteins and essential for the spore to withstand extreme environmental conditions and allow germination under favorable conditions. however, understanding how the properties of the coat arise from the interactions among all these proteins is an important challenge. moreover, many specific protein-protein interactions among the coat proteins are crucial for coat assembly. in this study, atomic force microscopy (afm) based single molecule force spectroscopy (smfs) was applied to investigate the interaction as a dynamic process between two morphogenetic coat proteins, cote and cotz. the unbinding force and kinetic parameters characterizing the interaction between cote and cotz were obtained. it is found that there is a strong affinity between cote and cotz. furthermore, the assembly behaviors of cote and cotz, individually or in combination, were studied by afm at solid-liquid interfaces. our results revealed that cote-cotz assembly is dependent on their molar ratios and the interaction between cote and cotz involves in the cote-cotz assembly. (c) 2016 elsevier inc. all rights reserved. |
| WOS关键词 | MOLECULAR RECOGNITION ; SPECTROSCOPY ; CELLS ; ENDOSPORE ; BINDING ; LAYER ; SAFA ; AFM ; VISUALIZATION ; LOCALIZATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000379452500011 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374660 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tang, Jilin |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Liaoning Univ Petr & Chem Technol, Coll Chem Chem Engn & Environm Engn, Fushun 113001, Peoples R China 4.Slovak Acad Sci, Inst Mol Biol, Dubrayska Cesta 21, Bratislava 84551, Slovakia |
| 推荐引用方式 GB/T 7714 | Liu, Huiqing,Qiao, Haiyan,Krajcikova, Daniela,et al. Physical interaction and assembly of bacillus subtilis spore coat proteins cote and cotz studied by atomic force microscopy[J]. Journal of structural biology,2016,195(2):245-251. |
| APA | Liu, Huiqing.,Qiao, Haiyan.,Krajcikova, Daniela.,Zhang, Zhe.,Wang, Hongda.,...&Tang, Jilin.(2016).Physical interaction and assembly of bacillus subtilis spore coat proteins cote and cotz studied by atomic force microscopy.Journal of structural biology,195(2),245-251. |
| MLA | Liu, Huiqing,et al."Physical interaction and assembly of bacillus subtilis spore coat proteins cote and cotz studied by atomic force microscopy".Journal of structural biology 195.2(2016):245-251. |
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来源：中国科学院大学
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