Study on the effect of eicosapentaenoic acid on phospholipids composition in membrane microdomains of tight junctions of epithelial cells by liquid chromatography/electrospray mass spectrometry
文献类型：期刊论文
| 作者 | Zhao, Sumin1; Jia, Lewen1; Gao, Peng1; Li, Qiurong2; Lu, Xin1; Li, Jieshou2; Xu, Guowang1 |
| 刊名 | journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2008-06-09 |
| 卷号 | 47期号:2页码:343-350 |
| 关键词 | eicosapentaenoic acid tight junction phospholipids HPLC-MS |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | tight junctions of epithelial cells determine epithelial membrane integrity and play an important role in selective paracellular permeability to ions and macromolecules. in this work, we investigated the effect of one of n-3 series polyunsaturated fatty acids, eicosapentaenoic acid (epa) on the phospholipid composition of membrane microdomains of tight junctions. after treated by epa, membrane microdomains of tight junctions were isolated by discontinuous sucrose density gradient ultracentrifugation, and raft phospholipids were extracted. the pe, pi, ps, pc and sm were separated and determined by high-performance liquid chromatography/quadrupole-linear ion trap mass spectrometry (hplc qtrap-ms), and were further identified by hplc-ms/ms. it was found that epa altered the fatty acyl substitution of phospholipids that constituted membrane microdomains of tight junctions by enriching the unsaturated fatty acyl chains of the phospholipids. it provides a new visual angle to explaining the intracellular mechanism involved in n-3 polyunsaturated fatty acids (pufas) modulation of intestinal tight junction barrier. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | polyunsaturated fatty-acids ; crohns-disease ; human blood ; intestinal permeability ; caco-2 cells ; identification ; proteins ; barrier |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000256649400016 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/100437]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China 2.Jinling Hosp, Inst Gen Surg, Nanjing 210002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Sumin,Jia, Lewen,Gao, Peng,et al. Study on the effect of eicosapentaenoic acid on phospholipids composition in membrane microdomains of tight junctions of epithelial cells by liquid chromatography/electrospray mass spectrometry[J]. journal of pharmaceutical and biomedical analysis,2008,47(2):343-350. |
| APA | Zhao, Sumin.,Jia, Lewen.,Gao, Peng.,Li, Qiurong.,Lu, Xin.,...&Xu, Guowang.(2008).Study on the effect of eicosapentaenoic acid on phospholipids composition in membrane microdomains of tight junctions of epithelial cells by liquid chromatography/electrospray mass spectrometry.journal of pharmaceutical and biomedical analysis,47(2),343-350. |
| MLA | Zhao, Sumin,et al."Study on the effect of eicosapentaenoic acid on phospholipids composition in membrane microdomains of tight junctions of epithelial cells by liquid chromatography/electrospray mass spectrometry".journal of pharmaceutical and biomedical analysis 47.2(2008):343-350. |
入库方式： OAI收割
来源：大连化学物理研究所
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