Structural basis of viral rna-dependent rna polymerase catalysis and translocation
文献类型：期刊论文
| 作者 | Shu, Bo1,2; Gong, Peng1 |
| 刊名 | Proceedings of the national academy of sciences of the united states of america
 |
| 出版日期 | 2016-07-12 |
| 卷号 | 113期号:28页码:E4005-e4014 |
| ISSN号 | 0027-8424 |
| 关键词 | Rna-dependent rna polymerase Nucleotide addition cycle Translocation intermediate Enterovirus 71 Crystal structure |
| DOI | 10.1073/pnas.1602591113 |
| 通讯作者 | Gong, peng(gongpeng@wh.iov.cn) |
| 英文摘要 | Viral rna-dependent rna polymerases (rdrps) play essential roles in viral genome replication and transcription. we previously reported several structural states of the poliovirus rdrp nucleotide addition cycle (nac) that revealed a unique palm domain-based active site closure mechanism and proposed a six-state nac model including a hypothetical state representing translocation intermediates. using the rdrp from another human enterovirus, enterovirus 71, here we report seven rdrp elongation complex structures derived from a crystal lattice that allows three nac events. these structures suggested a key order of events in initial ntp binding and ntp-induced active site closure and revealed a bona fide translocation intermediate featuring asymmetric movement of the template-product duplex. our work provides essential missing links in understanding ntp recognition and translocation mechanisms in viral rdrps and emphasizes the uniqueness of the viral rdrps compared with other processive polymerases. |
| WOS关键词 | II ELONGATION COMPLEX ; DNA-POLYMERASE ; REPLICATION FIDELITY ; NUCLEOTIDYL TRANSFER ; ESCHERICHIA-COLI ; ACTIVE-SITE ; TRANSCRIPTION ; VIRUS ; INITIATION ; MECHANISM |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | NATL ACAD SCIENCES |
| WOS记录号 | WOS:000379694100006 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374688 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gong, Peng |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shu, Bo,Gong, Peng. Structural basis of viral rna-dependent rna polymerase catalysis and translocation[J]. Proceedings of the national academy of sciences of the united states of america,2016,113(28):E4005-e4014. |
| APA | Shu, Bo,&Gong, Peng.(2016).Structural basis of viral rna-dependent rna polymerase catalysis and translocation.Proceedings of the national academy of sciences of the united states of america,113(28),E4005-e4014. |
| MLA | Shu, Bo,et al."Structural basis of viral rna-dependent rna polymerase catalysis and translocation".Proceedings of the national academy of sciences of the united states of america 113.28(2016):E4005-e4014. |
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来源：中国科学院大学
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