Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system
文献类型：期刊论文
| 作者 | Liu, Sha1,2; Zhang, Hongyan1; Liu, Weimin1; Zhou, Bingjiang1; Ma, Qian1,3; Ge, Jiechao1; Wu, Jiasheng1; Wang, Pengfei1 |
| 刊名 | Rsc advances
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:70页码:65930-65935 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c6ra10396k |
| 通讯作者 | Zhang, hongyan(zhanghongyan@mail.ipc.ac.cn) |
| 英文摘要 | In our work, we investigated the interactions between a small molecule, folic acid, and biological cells through the interaction of folic acid and folate receptors using a laser scanning confocal imaging-surface plasmon resonance (lsci-spr) system. the changes of spr peaks and cell concentration had good linear relationships, and fluorescence imaging provided further data. the detection limit was as low as 1.0 x 10(3) cells per ml, and linear coefficients were 0.95206, 0.95454, 0.94287, 0.98711, and 0.99228 for mouse lymphoma (l5178y tk+/-) cells, mouse lymphoma (el4) cells, mouse t lymphocytes (cl.ly 1+2-/9) cells, human lung cancer (a549) cells, and human oral epidermis carcinoma (kb) cells, respectively. the results indicated that the lsci-spr system has potential future application in analyzing small molecule-biological cell affinity and in acquiring quantitative parameters. |
| WOS关键词 | FOLIC-ACID ; CANCER-CELLS ; BREAST-CANCER ; RECEPTOR EXPRESSION ; ANTICANCER DRUGS ; FOLATE RECEPTORS ; BINDING-KINETICS ; DELIVERY ; PROTEIN ; AU |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000379577800076 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374711 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Hongyan |
| 作者单位 | 1.Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Univ Petr, Beijing Key Lab Opt Detect Technol Oil & Gas, Beijing 102249, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Sha,Zhang, Hongyan,Liu, Weimin,et al. Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system[J]. Rsc advances,2016,6(70):65930-65935. |
| APA | Liu, Sha.,Zhang, Hongyan.,Liu, Weimin.,Zhou, Bingjiang.,Ma, Qian.,...&Wang, Pengfei.(2016).Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system.Rsc advances,6(70),65930-65935. |
| MLA | Liu, Sha,et al."Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system".Rsc advances 6.70(2016):65930-65935. |
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来源：中国科学院大学
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