Morphologically and functionally distinct lipid droplet subpopulations
文献类型：期刊论文
| 作者 | Zhang, Shuyan1; Wang, Yang1; Cui, Liujuan1,2; Deng, Yaqin1,3; Xu, Shimeng1,3; Yu, Jinhai1,3; Cichello, Simon4; Serrero, Ginette5; Ying, Yunshu6; Liu, Pingsheng1 |
| 刊名 | Scientific reports
 |
| 出版日期 | 2016-07-08 |
| 卷号 | 6页码:14 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep29539 |
| 通讯作者 | Liu, pingsheng(pliu@ibp.ac.cn) |
| 英文摘要 | Lipid droplet (ld), a multi-functional organelle, is often found to associate with other cellular membranous structures and vary in size in a given cell, which may be related to their functional diversity. here we established a method to separate ld subpopulations from isolated cho k2 lds into three different size categories. the subpopulation with smallest lds was nearly free of er and other membranous structures while those with larger lds contained intact er. these distinct subpopulations of lds differed in their protein composition and ability to recruit proteins. this method was also applicable to lds obtained from other sources, such as huh7 cells, mouse liver and brown adipose tissue, et al. we developed an in vitro assay requiring only isolated lds, coenzyme a, and atp to drive lipid synthesis. the ld subpopulation nearly depleted of er was able to incorporate fatty acids into triacylglycerol and phospholipids. together, our data demonstrate that lds in a given cell are heterogeneous in size and function, and suggest that lds are one of cellular lipid synthetic organelles. |
| WOS关键词 | YEAST SACCHAROMYCES-CEREVISIAE ; ENDOPLASMIC-RETICULUM ; TRIACYLGLYCEROL SYNTHESIS ; OIL BODIES ; PROTEIN ; IDENTIFICATION ; MEMBRANE ; REVEALS ; CELLS ; LOCALIZATION |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000379769200001 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374721 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Pingsheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.La Trobe Univ, Sch Life Sci, Melbourne, Vic 3086, Australia 5.A&G Pharmaceut Inc, Columbia, MD 21045 USA 6.Univ Texas Southwestern Med Ctr, Dallas, TX 75390 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Shuyan,Wang, Yang,Cui, Liujuan,et al. Morphologically and functionally distinct lipid droplet subpopulations[J]. Scientific reports,2016,6:14. |
| APA | Zhang, Shuyan.,Wang, Yang.,Cui, Liujuan.,Deng, Yaqin.,Xu, Shimeng.,...&Liu, Pingsheng.(2016).Morphologically and functionally distinct lipid droplet subpopulations.Scientific reports,6,14. |
| MLA | Zhang, Shuyan,et al."Morphologically and functionally distinct lipid droplet subpopulations".Scientific reports 6(2016):14. |
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来源：中国科学院大学
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