A fluorescence turn on assay for alkaline phosphatase based on the cu2+ catalyzed fenton-like reaction
文献类型：期刊论文
| 作者 | Zhang, Qingfeng1,2; Zhang, Cuiyun1,2,3; Shahzad, Sohail Anjum1,4; Yu, Cong1,2 |
| 刊名 | Talanta
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 158页码:342-350 |
| 关键词 | Alkaline phosphatase Fenton reaction Fluorescence Single stranded dna Graphene oxide |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2016.05.067 |
| 通讯作者 | Yu, cong(congyu@ciac.ac.cn) |
| 英文摘要 | A fluorescence turn-on assay was established for alp (alkaline phosphatase) based on cu2+ catalyzed fenton-like reaction and graphene oxide (go). go was utilized to quench the fluorescence of fluorescein (fam) labeled single strand dna (f-dna). alp can remove the phosphate group in sodium ascorbyl phosphate (sap), and convert it into reducing ascorbate. highly reactive hydroxyl radicals (center dot oh) were generated in the presence of ascorbate and cu2+ through the fenton-like reaction. the reactive radicals generated in situ caused the cleavage of f-dna into small fragments. when go was added, the fluorescence emission of the sample without alp was quenched and fluorescence emission recovered in the presence of alp. the intensity of the recovered fluorescence was directly related to the concentration of alp in the assay solution, and a sensitive and selective facile alp assay is therefore established. (c) 2016 elsevier b.v. all rights reserved. |
| WOS关键词 | GRAPHENE OXIDE ; GOLD NANOPARTICLES ; DNA CLEAVAGE ; ON DETECTION ; PLATFORM ; INHIBITION ; STRATEGY ; DISEASES ; SYSTEM ; FE2+ |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000379370400045 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374726 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, Cong |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Tianjin Univ, Sch Mat Sci & Engn, Tianjin 300072, Peoples R China 4.COMSATS Inst Informat Technol, Dept Chem, Abbottabad 22060, Pakistan |
| 推荐引用方式 GB/T 7714 | Zhang, Qingfeng,Zhang, Cuiyun,Shahzad, Sohail Anjum,et al. A fluorescence turn on assay for alkaline phosphatase based on the cu2+ catalyzed fenton-like reaction[J]. Talanta,2016,158:342-350. |
| APA | Zhang, Qingfeng,Zhang, Cuiyun,Shahzad, Sohail Anjum,&Yu, Cong.(2016).A fluorescence turn on assay for alkaline phosphatase based on the cu2+ catalyzed fenton-like reaction.Talanta,158,342-350. |
| MLA | Zhang, Qingfeng,et al."A fluorescence turn on assay for alkaline phosphatase based on the cu2+ catalyzed fenton-like reaction".Talanta 158(2016):342-350. |
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来源：中国科学院大学
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