Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis
文献类型：期刊论文
| 作者 | Gao, Peng2; Lu, Chen1; Zhang, Fengxia2,4; Sang, Ping2,3; Yang, Dawei2,3; Li, Xiang2; Kong, Hongwei2; Yin, Peiyuan2; Tian, Jing2,3; Lu, Xin2 |
| 刊名 | analyst
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 133期号:9页码:1214-1220 |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | ankylosing spondylitis (as) is a chronic inflammatory arthritis that predominantly affects the axial skeleton in adolescent patients. the natural history of the disease remains poorly characterized. in this study, we combined gc-ms and lc-ms techniques to evaluate the major metabolic changes in the plasma of as patients in view of metabonomics. univariate and multivariate analysis were employed for altered metabolite comparison and pattern recognition. application of supervised partial least-squares discrminant analysis to either gc-ms or lc-ms data allowed accurate discrimination of as patients from normal controls, demonstrating its potential diagnostic utilization. in addition, as patients presented elevated plasma concentrations of proline, glucose, phosphate, urea, glycerol, phenylalanine and homocysteine but reduced levels of phosphocholines, tryptophan and a bipeptide - phenylalanyl-phenylalanine. in the context of their involved metabolic pathways, the identified metabolites were discussed accordingly. this investigation primarily proved that integrated chromatography-mass spectrometry and integrated uni- and multi-variate statistical analysis facilitated metabonomics to be a more promising tool in disease research. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | chromatography-mass spectrometry ; research-and-development ; bone-mineral density ; systems biology ; metabolic interactions ; rheumatoid-arthritis ; biochemical markers ; endothelial-cells ; gene-expression ; cultured human |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000259449400014 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/100453]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing 100700, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 3.Dalian Polytech Univ, Dept Modern Technol, Dalian 116034, Peoples R China 4.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Peng,Lu, Chen,Zhang, Fengxia,et al. Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis[J]. analyst,2008,133(9):1214-1220. |
| APA | Gao, Peng.,Lu, Chen.,Zhang, Fengxia.,Sang, Ping.,Yang, Dawei.,...&Xu, Guowang.(2008).Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis.analyst,133(9),1214-1220. |
| MLA | Gao, Peng,et al."Integrated GC-MS and LC-MS plasma metabonomics analysis of ankylosing spondylitis".analyst 133.9(2008):1214-1220. |
入库方式： OAI收割
来源：大连化学物理研究所
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