A reliable compound-specific nitrogen isotope analysis of amino acids by gc-c-irms following derivatisation into n-pivaloyl-iso-propyl (npip)esters for high-resolution food webs estimation
文献类型：期刊论文
| 作者 | Zhang, Zhongyi1,2; Tian, Jing1,2; Xiao, Hongwei3; Zheng, Nengjian1,2; Gao, Xiaofei2,4; Zhu, Renguo1,3; Xiao, Huayun1 |
| 刊名 | Journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2016-10-15 |
| 卷号 | 1033页码:382-389 |
| 关键词 | Gc-c-irms Amino acid Trophic level Npip ester |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2016.09.004 |
| 通讯作者 | Xiao, huayun(huayunxiao@outlook.com) |
| 英文摘要 | The signatures of natural stable nitrogen isotopic composition (delta n-15) of individual amino acid (aa) have been confirmed to be a potentially effective tool for elucidating nitrogen cycling and trophic position of various organisms in food webs. in the present study, a two-stage derivatisation approach of esterification followed by acylation was evaluated. the biological samples underwent acid hydrolysis and the released individual aa was derivatived into corresponding n-pivaloyl-isopropyl (npip) esters for nitrogen isotopic analysis in gas chromatography-combustion-isotope ratio mass spectrometry (gc-c-irms). usually, 13 individual aa derivatives were separated with fine baseline resolution based on a nonpolar gas chromatography column (db-5 ms). the minimum sample amount required under the presented conditions is larger than 20 ngn on column in order to accurately determine the delta n-15 values. the delta n-15 values determined by gc-c-irms with a precision of better than 1 parts per thousand, were within 1 parts per thousand after empirical correction compared to the corresponding measured by element analysis (ea)-irms. bland-altman plot showed highly consistency of the delta n-15 values determined by the two measurement techniques. cation exchange chromatography was applied to remove interfering fraction from the extracts of plant and animal samples and without nitrogen isotope fractionation during the treatment procedure. moreover, this approach was carried out to estimate the trophic level of various natural organisms in a natural lake environment. results highly proved that the trophic level estimated via the presented aa method well reflected the actual food web structure in natural environments. (c) 2016 elsevier b.v. all rights reserved. |
| WOS关键词 | RATIO MASS-SPECTROMETRY ; DELTA-C-13 VALUES ; DELTA-N-15 VALUES ; CHROMATOGRAPHY ; LIQUID ; DISCRIMINATION ; OPTIMIZATION ; PERFORMANCE ; SAMPLES ; CARBON |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000385322600048 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374767 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xiao, Huayun |
| 作者单位 | 1.Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550081, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.East China Univ Technol, Atmospher Environm Lab, Key Lab Nucl Resource & Environm, Minist Educ, Nanchang 330013, Peoples R China 4.Chinese Acad Sci, Inst Urban Environm, Aquat Ecohlth Grp, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhongyi,Tian, Jing,Xiao, Hongwei,et al. A reliable compound-specific nitrogen isotope analysis of amino acids by gc-c-irms following derivatisation into n-pivaloyl-iso-propyl (npip)esters for high-resolution food webs estimation[J]. Journal of chromatography b-analytical technologies in the biomedical and life sciences,2016,1033:382-389. |
| APA | Zhang, Zhongyi.,Tian, Jing.,Xiao, Hongwei.,Zheng, Nengjian.,Gao, Xiaofei.,...&Xiao, Huayun.(2016).A reliable compound-specific nitrogen isotope analysis of amino acids by gc-c-irms following derivatisation into n-pivaloyl-iso-propyl (npip)esters for high-resolution food webs estimation.Journal of chromatography b-analytical technologies in the biomedical and life sciences,1033,382-389. |
| MLA | Zhang, Zhongyi,et al."A reliable compound-specific nitrogen isotope analysis of amino acids by gc-c-irms following derivatisation into n-pivaloyl-iso-propyl (npip)esters for high-resolution food webs estimation".Journal of chromatography b-analytical technologies in the biomedical and life sciences 1033(2016):382-389. |
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来源：中国科学院大学
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