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An oyster species-specific mirna scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway

文献类型：期刊论文

作者Chen, Hao1,3; Wang, Hao1; Jiang, Shuai1; Xu, Jiachao1,3; Wang, Lingling2; Qiu, Limei1; Song, Linsheng2
刊名Fish & shellfish immunology
出版日期2016-10-01
卷号57页码:160-169
关键词Species-specific mirna Cellular immune response Integrin-linked kinase Integrin pathway Cell migration
ISSN号1050-4648
DOI10.1016/j.fsi.2016.08.032
通讯作者Wang, hao(haowang@qdio.ac.cn) ; Song, linsheng(lshsong@dlou.edu.cn)
英文摘要Mirnas are important gene regulators at post-transcriptional level and can modulate diverse biological processes, including immune response. dozens of species-specific mirnas have been identified in oyster crassostrea gigas while their functions remain largely unknown. in the present study, an oyster species specific mirna scaffold42648_5080 was found responsive to lps stimulation and might target a total of 31 oyster genes possibly involved in cell communication, cellular localization and cellular response to stimulus. besides, in gain-of-function assay of scaffold42648_5080 in vivo, the phagocytosis (30.90% in mirna group verse 23.20% in mirna control group), apoptosis (3.10% in mirna group verse 5.30% in mirna control group) and migration rate (13.88% in mirna group verse 21.03% in mirna control group) of oyster haemocytes were found significantly altered after the injection of scaffold42648_5080 mimics. among the target genes, integrin-linked kinase (cgilk) was considered crucial in cell migration and its interaction with scaffold42648_5080 was then verified both in vitro and in vivo. consequently, a significant decrease of relative luciferase ratio was observed in cgilk 3'-utr luciferase reporter assay after transfection of scaffold42648_5080 mimics (0.70-fold of that in blank group, p < 0.01). meanwhile, when scaffold42648_5080 was overexpressed in vivo (5.41-fold of mirna control group, p < 0.01), the expression of cgilk declined significantly to 0.25-fold of mirna control group (p < 0.01). comparatively, a significant decrease of the haemocyte migration rate (19.76% verse 34.82% in siegfp control group, p < 0.01) was observed after knock-down of cgilk in vivo. the present study, as far as we know, for the first time revealed the immunomodulation role of an oyster species-specific mirna, which might provide new insights into mirna-mediated adaptation mechanism of oysters. (c) 2016 elsevier ltd. all rights reserved.
WOS关键词DEEP SEQUENCING DATA ; CRASSOSTREA-GIGAS ; MICRORNA GENES ; VIBRIO-SPLENDIDUS ; BINDING-ACTIVITY ; INNATE IMMUNITY ; CELL-MIGRATION ; EVOLUTION ; IDENTIFICATION ; NOREPINEPHRINE
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000385327100017
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
URI标识http://www.irgrid.ac.cn/handle/1471x/2374800
专题中国科学院大学
通讯作者Wang, Hao; Song, Linsheng
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Hao,Wang, Hao,Jiang, Shuai,et al. An oyster species-specific mirna scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway[J]. Fish & shellfish immunology,2016,57:160-169.
APA Chen, Hao.,Wang, Hao.,Jiang, Shuai.,Xu, Jiachao.,Wang, Lingling.,...&Song, Linsheng.(2016).An oyster species-specific mirna scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway.Fish & shellfish immunology,57,160-169.
MLA Chen, Hao,et al."An oyster species-specific mirna scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway".Fish & shellfish immunology 57(2016):160-169.

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