Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications
文献类型：期刊论文
| 作者 | Jiang, Su1; Liu, Ya-Feng2,3; Wang, Xiao-Min4; Liu, Ke-Fei5; Zhang, Ding-Hong5,6; Li, Yi-Ding5,7; Yu, Ai-Ping1; Zhang, Xiao-Hui7; Zhang, Jia-Yi8,9; Xu, Jian-Guang1,10 |
| 刊名 | Biomedical optics express
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 7期号:9页码:3478-3490 |
| ISSN号 | 2156-7085 |
| DOI | 10.1364/boe.7.003478 |
| 通讯作者 | Xu, wen-dong(wendongxu@fudan.edu.cn) |
| 英文摘要 | We introduce a more flexible optogenetics-based mapping system attached on a stereo microscope, which offers automatic light stimulation to individual regions of interest in the cortex that expresses light-activated channelrhodopsin-2 in vivo. combining simultaneous recording of electromyography from specific forelimb muscles, we demonstrate that this system offers much better efficiency and precision in mapping distinct domains for controlling limb muscles in the mouse motor cortex. furthermore, the compact and modular design of the system also yields a simple and flexible implementation to different commercial stereo microscopes, and thus could be widely used among laboratories. (c) 2016 optical society of america |
| WOS关键词 | MOTOR CORTEX ; TRANSGENIC MICE ; 2-PHOTON EXCITATION ; NEURAL ACTIVITY ; CHANNELRHODOPSIN-2 ; STIMULATION ; CONNECTIVITY ; ACTIVATION ; MOVEMENT ; SYSTEMS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
| WOS类目 | Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000385416500026 |
| 出版者 | OPTICAL SOC AMER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374801 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xu, Wen-Dong |
| 作者单位 | 1.Fudan Univ, Shanghai Med Coll, Huashan Hosp, Dept Hand Surg, Shanghai 200040, Peoples R China 2.Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect, Wuhan 430074, Peoples R China 3.Huazhong Univ Sci & Technol, Dept Biomed Engn, MoE Key Lab Biomed Photon, Wuhan 430074, Peoples R China 4.Jing Dist Cent Hosp, Dept Hand & Upper Extrem Surg, Shanghai 200040, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China 6.Grad Univ Chinese Acad Sci, Shanghai 200031, Peoples R China 7.Beijing Normal Univ, IDG McGovern Inst Brain Res, State Key Lab Cognit Neurosci & Learning, Beijing 100875, Peoples R China 8.Fudan Univ, Inst Brain Sci, Shanghai 200031, Peoples R China 9.Fudan Univ, Collaborat Innovat Ctr Brain Sci, State Key Lab Med Neurobiol, Shanghai 200031, Peoples R China 10.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Su,Liu, Ya-Feng,Wang, Xiao-Min,et al. Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications[J]. Biomedical optics express,2016,7(9):3478-3490. |
| APA | Jiang, Su.,Liu, Ya-Feng.,Wang, Xiao-Min.,Liu, Ke-Fei.,Zhang, Ding-Hong.,...&Zeng, Shao-Qun.(2016).Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications.Biomedical optics express,7(9),3478-3490. |
| MLA | Jiang, Su,et al."Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications".Biomedical optics express 7.9(2016):3478-3490. |
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来源：中国科学院大学
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