Ppar gamma is regulated by mir-27b-3p negatively and plays an important role in porcine oocyte maturation
文献类型：期刊论文
| 作者 | Song, Chunlei1; Yao, Jing2,3; Cao, Chunwei2,3; Liang, Xiaojuan1; Huang, Jiaojiao2,3; Han, Ziqiang2,4; Zhang, Yang2,3; Qin, Guosong2,3; Tao, Cong1; Li, Chengbo5 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2016-10-14 |
| 卷号 | 479期号:2页码:224-230 |
| ISSN号 | 0006-291X |
| 关键词 | Pig Mirna Oocyte maturation Ppar gamma Fatty acid metabolism |
| DOI | 10.1016/j.bbrc.2016.09.046 |
| 通讯作者 | Wang, yanfang(wangyanfang@caas.cn) |
| 英文摘要 | To elucidate the key mirnas and the signalling pathways that are involved in porcine oocyte maturation, we performed a deep sequencing analysis of the mirnas of pig germinal vesicle (gv) oocytes and metaphase ii (mii) oocytes. seven differentially expressed (de) mirnas were identified and the expression levels of mir-21 and mir-27b-3p were further confirmed by qpcr analysis. the target genes of 7 de mirnas were predicted and subjected to pathway analysis. interestingly, fatty acid metabolism and fatty acid biosynthesis were the top two significantly enriched molecular functions during oocyte maturation. heat map, which was built with 7 de mirnas and the enriched the molecular functions, revealed that mir-21, mir-27b-3p, mir-10a-5p and mir-10b-5p were involved in fatty acid metabolism. in particular, the regulatory role of mir-27b-3p on peroxisome proliferator-activated receptor-gamma (ppar gamma) was confirmed by their inversed expression patterns in gv and mii oocytes and luciferase report assays. in addition, we observed that ppar gamma agonist (rosiglitazone) treatment significantly enhanced porcine oocyte maturation rate and early embryo developmental competent. taken together, our results demonstrated that mir-27b and its target, ppar gamma, play the vital roles in pig oocyte maturation through regulating the fatty acid metabolism. these data increased our understanding of the regulatory gene networks in porcine oocyte maturation and development. (c) 2016 elsevier inc. all rights reserved. |
| WOS关键词 | EXPRESSION ; TARGETS ; LIPIDS ; CELLS ; MICE ; PIG |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000385898900017 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374806 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Yanfang |
| 作者单位 | 1.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100081, Peoples R China 2.Chinese Acad Sci, State Key Lab Reprod Biol, Inst Zool, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Cunji Med Sch, Beijing, Peoples R China 4.Hebei Univ, Coll Life Sci, Baoding 071000, Peoples R China 5.Rongchang Breeding Co, Binzhou 251900, Shandong, Peoples R China 6.Tsinghua Univ, High Sch, Beijing 100094, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Chunlei,Yao, Jing,Cao, Chunwei,et al. Ppar gamma is regulated by mir-27b-3p negatively and plays an important role in porcine oocyte maturation[J]. Biochemical and biophysical research communications,2016,479(2):224-230. |
| APA | Song, Chunlei.,Yao, Jing.,Cao, Chunwei.,Liang, Xiaojuan.,Huang, Jiaojiao.,...&Wang, Yanfang.(2016).Ppar gamma is regulated by mir-27b-3p negatively and plays an important role in porcine oocyte maturation.Biochemical and biophysical research communications,479(2),224-230. |
| MLA | Song, Chunlei,et al."Ppar gamma is regulated by mir-27b-3p negatively and plays an important role in porcine oocyte maturation".Biochemical and biophysical research communications 479.2(2016):224-230. |
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来源：中国科学院大学
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