Single-molecule visualization of dynamic transitions of pore-forming peptides among multiple transmembrane positions
文献类型：期刊论文
| 作者 | Li, Ying1,2; Qian, Zhenyu3,4; Ma, Li1,2; Hu, Shuxin1,2; Nong, Daguan1,2; Xu, Chunhua1,2; Ye, Fangfu1,2; Lu, Ying1,2; Wei, Guanghong3,4; Li, Ming1,2,5 |
| 刊名 | Nature communications
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 7页码:9 |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/ncomms12906 |
| 通讯作者 | Lu, ying(yinglu@iphy.ac.cn) ; Wei, guanghong(ghwei@fudan.edu.cn) ; Li, ming(mingli@iphy.ac.cn) |
| 英文摘要 | Research on the dynamics of single-membrane proteins remains underdeveloped due to the lack of proper approaches that can probe in real time the protein's insertion depth in lipid bilayers. here we report a single-molecule visualization method to track both vertical insertion and lateral diffusion of membrane proteins in supported lipid bilayers by exploiting the surface-induced fluorescence attenuation (sifa) of fluorophores. the attenuation follows a d(-4) dependency, where d is the fluorophore-to-surface distance. the method is validated by observing the antimicrobial peptide ll-37 to transfer among five transmembrane positions: the surface, the upper leaflet, the centre, the lower leaflet and the bottom of the lipid bilayer. these results demonstrate the power of sifa to study protein-membrane interactions and provide unprecedented in-depth understanding of molecular mechanisms of the insertion and translocation of membrane proteins. |
| WOS关键词 | HUMAN ANTIMICROBIAL PEPTIDE ; HUMAN CATHELICIDIN LL-37 ; GRAPHENE OXIDE ; LIPID-BILAYERS ; MEMBRANE INTERACTIONS ; ENERGY-TRANSFER ; FORCE-FIELD ; FLUORESCENCE ; PROTEINS ; SHEETS |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000385387700002 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374811 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Lu, Ying; Wei, Guanghong; Li, Ming |
| 作者单位 | 1.Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Phys, CAS Key Lab Soft Matter Phys, Beijing 100190, Peoples R China 3.Fudan Univ, State Key Lab Surface Phys, Key Lab Computat Phys Sci, Minist Educ, Shanghai 200433, Peoples R China 4.Fudan Univ, Dept Phys, Shanghai 200433, Peoples R China 5.Univ Chinese Acad Sci, Sch Phys Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ying,Qian, Zhenyu,Ma, Li,et al. Single-molecule visualization of dynamic transitions of pore-forming peptides among multiple transmembrane positions[J]. Nature communications,2016,7:9. |
| APA | Li, Ying.,Qian, Zhenyu.,Ma, Li.,Hu, Shuxin.,Nong, Daguan.,...&Li, Ming.(2016).Single-molecule visualization of dynamic transitions of pore-forming peptides among multiple transmembrane positions.Nature communications,7,9. |
| MLA | Li, Ying,et al."Single-molecule visualization of dynamic transitions of pore-forming peptides among multiple transmembrane positions".Nature communications 7(2016):9. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。