Ef4 reveals the energy barrier for trna back-translocation in the peptidyl transferase center
文献类型：期刊论文
| 作者 | Song, Guangtao1; Qin, Yan1,2 |
| 刊名 | Rna biology
 |
| 出版日期 | 2016 |
| 卷号 | 13期号:10页码:934-939 |
| 关键词 | Back-translocation Ef4 Energy barrier Ptc Ribosome Trna |
| ISSN号 | 1547-6286 |
| DOI | 10.1080/15476286.2016.1215795 |
| 通讯作者 | Qin, yan(qiny@ibp.ac.cn) |
| 英文摘要 | In the translating ribosomal complex, transfer rna (trna) is stabilized in the ribosome by its anticodon stem-loop (asl) and 3-cca end through base-pairing interactions with mrna codon on the small subunit and rrna in the peptidyl transferase center (ptc) of large subunit, respectively.elongation factor 4 (ef4), a highly conserved translational gtpase, has been identified to trigger back-translocation. early this year, we reported high resolution cryo-em structures of ef4 in complex with escherichia coli 70s ribosome in pre- and post-translocational states with direct observations that ef4 disrupts the base pairs between the 3-end of peptidyl-trna and the p-loop of rrna in ptc. here, we focus on the novel molecular mechanism how ef4 catalyzes back-translocation, and discuss the common and specific energy barriers for forward- and back-translocation. |
| WOS关键词 | ELONGATION-FACTOR ; PROTEIN-SYNTHESIS ; CRYSTAL-STRUCTURE ; RIBOSOME ; TRANSLATION ; DYNAMICS ; LEPA ; GTPASE ; GUF1 |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000385561700002 |
| 出版者 | TAYLOR & FRANCIS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374865 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qin, Yan |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Key Lab RNA Biol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Guangtao,Qin, Yan. Ef4 reveals the energy barrier for trna back-translocation in the peptidyl transferase center[J]. Rna biology,2016,13(10):934-939. |
| APA | Song, Guangtao,&Qin, Yan.(2016).Ef4 reveals the energy barrier for trna back-translocation in the peptidyl transferase center.Rna biology,13(10),934-939. |
| MLA | Song, Guangtao,et al."Ef4 reveals the energy barrier for trna back-translocation in the peptidyl transferase center".Rna biology 13.10(2016):934-939. |
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来源：中国科学院大学
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