Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish cyanea nozakii
文献类型：期刊论文
| 作者 | Li, Rongfeng1; Yu, Huahua1; Yue, Yang1,2; Liu, Song1; Xing, Ronge1; Chen, Xiaolin1; Li, Pengcheng1 |
| 刊名 | Journal of proteomics
 |
| 出版日期 | 2016-10-04 |
| 卷号 | 148页码:57-64 |
| 关键词 | Jellyfish Cyanea nozakii Toxin Venom Transcriptomics Proteomics |
| ISSN号 | 1874-3919 |
| DOI | 10.1016/j.jprot.2016.07.023 |
| 通讯作者 | Li, pengcheng(pcli@gdio.ac.cn) |
| 英文摘要 | Jellyfish sting has become a worldwide issue of critical concern to human health and safety in coastal areas in recent decades. cyanea nozakii is one of the dominant blooming species and dangerous stingers in china. however, it remains unclear how many and what types of toxins are present in the venom. so, we used a combined transcriptomics and proteomics approach to investigate the venom composition of jellyfish c. nozakii. in total 4,608,524 illumina valid reads were obtained to de novo assemble to 40,434 unigenes in the transcriptomics analysis. and, a total of 311,635 ms/ms spectra with 12,247 unique ms/ms spectra were generated to 1556 homologous proteins in the proteomics analysis. 174 potential toxin proteins were identified, with 27 proteins homology to the toxins from venomous animals, including phospholipase a2, zinc metalloproteinase-disintegrin agkistin, serine protease inhibitor, plancitoxin-1, alpha-latrocrustotoxin-ltla, etc. this study described the transcriptomics and venom proteomics of jellyfish c nozakii for the first time. our findings provide a comprehensive understanding of the venom composition of c nozakii. furthermore, the results may also be very helpful for the discovery of novel bioactive proteins, as well as the development of effective treatments for jellyfish sting in the future. biological significance: jellyfish cyanea nozakii is one of the most dangerous stingers in the coast of china. hundreds of thousands of people would be stung every year and victims suffered a severe pain, itch, swelling, inflammation, wheal and even more serious consequence. however, it remains unclear how many and what types of toxins are present as well as the relationship between the clinical symptoms and toxins. our combined transcriptomics and proteomics findings can provide a comprehensive understanding of the venom composition of c nozakii and will also be helpful for the development of effective treatments for jellyfish sting in the future. (c) 2016 published by elsevier b.v. |
| WOS关键词 | STOMOLOPHUS-MELEAGRIS ; NEMATOCYST VENOM ; CHIROPSALMUS-QUADRIGATUS ; ENVENOMATION SYNDROMES ; PARTIAL-PURIFICATION ; HEMOLYTIC-ACTIVITY ; THAI WATERS ; HABU-KURAGE ; SHK TOXIN ; PROTEINS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods |
| 语种 | 英语 |
| WOS记录号 | WOS:000385599700007 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374920 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Pengcheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, 19 Yuguan Rd, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Rongfeng,Yu, Huahua,Yue, Yang,et al. Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish cyanea nozakii[J]. Journal of proteomics,2016,148:57-64. |
| APA | Li, Rongfeng.,Yu, Huahua.,Yue, Yang.,Liu, Song.,Xing, Ronge.,...&Li, Pengcheng.(2016).Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish cyanea nozakii.Journal of proteomics,148,57-64. |
| MLA | Li, Rongfeng,et al."Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish cyanea nozakii".Journal of proteomics 148(2016):57-64. |
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来源：中国科学院大学
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