Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications
文献类型：期刊论文
| 作者 | Lou, Ruyun1,2; Yu, Weiting1; Song, Yizhe1,2; Ren, Ying1,2; Zheng, Huizhen1,2; Guo, Xin1; Lin, Yunfei4; Pan, Guoyu4; Wang, Xiuli3; Ma, Xiaojun1 |
| 刊名 | Carbohydrate polymers
 |
| 出版日期 | 2017-01-02 |
| 卷号 | 155页码:456-465 |
| 关键词 | Galactosylated alginate Hydroxyl groups Microcapsules Primary hepatocytes Biological artificial liver support systems |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2016.08.098 |
| 通讯作者 | Yu, weiting(yuwt@dicp.ac.cn) ; Wang, xiuli(panpan1210@dicp.ac.cn) |
| 英文摘要 | Galactose moieties are covalently coupled with sodium alginate to enhance liver-specific functions in microcapsules owing to the specific interaction between the galactose moieties and the asialoglycoprotein receptors (asgprs) of hepatocytes. in this study, galactosylated alginate (l-nh2-oh-alginate) based microcapsules with desirable stability and a suitable 3d microenvironment are designed and fabricated for primary hepatocyte applications. the designed l-nh2-oh-alginate is fabricated via the application of ethylenediamine grafted lactobionic acid (l-nh2) onto the hydroxyl groups of sodium alginate so that the negatively charged carboxyl groups intact in l-nh2-oh-alginate can effectively bond with ca2+ to form a stable three-dimensional gel network; a subsequent reaction with polycations forms a stable membrane of microcapsules. as a result, l-nh2-oh-alginate based microcapsules exhibit an excellent mechanical stability. moreover, with a higher degree of substitution in l-nh2-oh-alginate (ds 0.41), the hepatocytes entrapped in l-nh2-oh-alginate microcapsules exhibit better viability and well-maintained liver-specific functions. (c) 2016 elsevier ltd. all rights reserved. |
| WOS关键词 | MICROENCAPSULATED HEPATOCYTES ; MECHANICAL STABILITY ; EXTRACELLULAR-MATRIX ; BIOARTIFICIAL LIVER ; CULTURE ; CHITOSAN ; RAT ; ENCAPSULATION ; OPTIMIZATION ; SUBSTRATUM |
| WOS研究方向 | Chemistry ; Polymer Science |
| WOS类目 | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000386402600050 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374929 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, Weiting; Wang, Xiuli |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Med Univ, Dept Histol & Embryol, Coll Basic Med Sci, Dalian 116044, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lou, Ruyun,Yu, Weiting,Song, Yizhe,et al. Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications[J]. Carbohydrate polymers,2017,155:456-465. |
| APA | Lou, Ruyun.,Yu, Weiting.,Song, Yizhe.,Ren, Ying.,Zheng, Huizhen.,...&Ma, Xiaojun.(2017).Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications.Carbohydrate polymers,155,456-465. |
| MLA | Lou, Ruyun,et al."Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications".Carbohydrate polymers 155(2017):456-465. |
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来源：中国科学院大学
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