Expression and characterization of a fibrinogenolytic enzyme from horsefly salivary gland
文献类型：期刊论文
| 作者 | Sheng, Xun1; Gao, Li2; Lu, Xiancui2,3; Wang, Yunfei2,3; Han, Yalan2,3; Meng, Ping2,4,5; Chen, Wenlin6; Lu, Qiumin2,4,5 |
| 刊名 | Protein expression and purification
 |
| 出版日期 | 2017 |
| 卷号 | 129页码:135-142 |
| 关键词 | Horsefly Salivary gland Fibrinogenolytic enzyme Anticoagulant Scp domain |
| ISSN号 | 1046-5928 |
| DOI | 10.1016/j.pep.2016.04.014 |
| 通讯作者 | Chen, wenlin(chenwenlin@aliyun.com) ; Lu, qiumin(lvqm@mail.kiz.ac.cn) |
| 英文摘要 | Enzymes from various natural resources are valuable in management of thrombosis. blood-sucking arthropods are one of these resources because they have a wide array of anti-hemostasis molecules in their salivary gland. however, it is difficult to purify enough protein samples from the salivary glands for pharmacological studies. in this work, a fibrinogenolytic enzyme (tablysin 2) identified from salivary glands of the horsefly tabanus yao was expressed in escherichia coli to further study its biological activities. the primary structure of tablysin 2 showed significant domain similarity to arthropod proteins from the antigen 5 family containing scp domain, whose biological functions are poorly understood. tablysin 2 cleaved the a alpha and part of b beta chains of fibrinogen and did not affect gamma chain and fibrin. it inhibited platelet aggregation induced by adp. it did not directly induce hemorrhage or activate plasminogen. the fibrinogenolytic activity of tablysin 2 provides a clue for the functions of antigen 5-related proteins in other haematophagous arthropods. this work demonstrate a method of expression of arthropod salivary proteins which are difficult to obtain from natural resources for further functional studies. (c) 2016 elsevier inc. all rights reserved. |
| WOS关键词 | BLOOD-FEEDING ARTHROPODS ; PLATELET-AGGREGATION ; VENOM ; PROTEINS ; PATHOGENESIS ; THROMBOSIS ; PURIFICATION ; HEMOSTASIS ; PROTEOMICS ; DIVERSITY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000386194500018 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374982 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Wenlin; Lu, Qiumin |
| 作者单位 | 1.Kunming Med Coll, Affiliated Stomatol Hosp, Kunming 650221, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Zool, Chinese Acad Sci & Yunnan Prov, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Yunnan, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100009, Peoples R China 4.Univ Sci & Technol China, Chinese Acad Sci, Kunming Inst Zool, United Lab Nat Peptide, Kunming 650223, Yunnan, Peoples R China 5.Chinese Acad Sci, Sinoafrican Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 6.Kunming Med Coll, Affiliated Hosp 3, Yunnan Clin Res Ctr Breast Canc, Kunming 650032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sheng, Xun,Gao, Li,Lu, Xiancui,et al. Expression and characterization of a fibrinogenolytic enzyme from horsefly salivary gland[J]. Protein expression and purification,2017,129:135-142. |
| APA | Sheng, Xun.,Gao, Li.,Lu, Xiancui.,Wang, Yunfei.,Han, Yalan.,...&Lu, Qiumin.(2017).Expression and characterization of a fibrinogenolytic enzyme from horsefly salivary gland.Protein expression and purification,129,135-142. |
| MLA | Sheng, Xun,et al."Expression and characterization of a fibrinogenolytic enzyme from horsefly salivary gland".Protein expression and purification 129(2017):135-142. |
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来源：中国科学院大学
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