A survival strategy for pseudomonas aeruginosa that uses exopolysaccharides to sequester and store iron to stimulate psl-dependent biofilm formation
文献类型：期刊论文
| 作者 | Yu, Shan1,2; Wei, Qing1; Zhao, Tianhu1,2; Guo, Yuan3; Ma, Luyan Z.1 |
| 刊名 | Applied and environmental microbiology
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 82期号:21页码:6403-6413 |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/aem.01307-16 |
| 通讯作者 | Ma, luyan z.(luyanma27@im.ac.cn) |
| 英文摘要 | Exopolysaccharide psl is a critical biofilm matrix component in pseudomonas aeruginosa, which forms a fiber-like matrix to enmesh bacterial communities. iron is important for p. aeruginosa biofilm development, yet it is not clearly understood how iron contributes to biofilm development. here, we showed that iron promoted biofilm formation via elevating psl production in p. aeruginosa. the high level of iron stimulated the synthesis of psl by reducing rhamnolipid biosynthesis and inhibiting the expression of amrz, a repressor of psl genes. iron-stimulated psl biosynthesis and biofilm formation held true in mucoid p. aeruginosa strains. subsequent experiments indicated that iron bound with psl in vitro and in biofilms, which suggested that psl fibers functioned as an iron storage channel in p. aeruginosa biofilms. moreover, among three matrix exopolysaccharides of p. aeruginosa, psl is the only exopolysaccharide that can bind with both ferrous and ferric ion, yet with higher affinity for ferrous iron. our data suggest a survival strategy of p. aeruginosa that uses exopolysaccharide to sequester and store iron to stimulate psl-dependent biofilm formation. importance pseudomonas aeruginosa is an environmental microorganism which is also an opportunistic pathogen that can cause severe infections in immunocompromised individuals. it is the predominant airway pathogen causing morbidity and mortality in individuals affected by the genetic disease cystic fibrosis (cf). increased airway iron and biofilm formation have been proposed to be the potential factors involved in the persistence of p. aeruginosa in cf patients. here, we showed that a high level of iron enhanced the production of the key biofilm matrix exopolysaccharide psl to stimulate psl-dependent biofilm formation. our results not only make the link between biofilm formation and iron concentration in cf, but also could guide the administration or use of iron chelators to interfere with biofilm formation in p. aeruginosa in cf patients. furthermore, our data also imply a survival strategy of p. aeruginosa under high-iron environmental conditions. |
| WOS关键词 | MATRIX POLYSACCHARIDE PSL ; DNA-BINDING PROTEIN ; CYSTIC-FIBROSIS ; SWARMING MOTILITY ; MUCOID PSEUDOMONAS ; BIOSURFACTANT SYNTHESIS ; OPPORTUNISTIC PATHOGEN ; TWITCHING MOTILITY ; BACTERIAL BIOFILMS ; ROLES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000386072300012 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2374990 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ma, Luyan Z. |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Tubingen, Ctr Appl Geosci, Tubingen, Germany |
| 推荐引用方式 GB/T 7714 | Yu, Shan,Wei, Qing,Zhao, Tianhu,et al. A survival strategy for pseudomonas aeruginosa that uses exopolysaccharides to sequester and store iron to stimulate psl-dependent biofilm formation[J]. Applied and environmental microbiology,2016,82(21):6403-6413. |
| APA | Yu, Shan,Wei, Qing,Zhao, Tianhu,Guo, Yuan,&Ma, Luyan Z..(2016).A survival strategy for pseudomonas aeruginosa that uses exopolysaccharides to sequester and store iron to stimulate psl-dependent biofilm formation.Applied and environmental microbiology,82(21),6403-6413. |
| MLA | Yu, Shan,et al."A survival strategy for pseudomonas aeruginosa that uses exopolysaccharides to sequester and store iron to stimulate psl-dependent biofilm formation".Applied and environmental microbiology 82.21(2016):6403-6413. |
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来源：中国科学院大学
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