One-pot synthesis of quencher labeled hairpin dna-cdte qds conjugate for target dna and deoxyribonuclease i detection
文献类型：期刊论文
| 作者 | Zhang, Qingfeng1,2; Li, Juanmin1,2; Zhang, Yunyi1,2,3; Hu, Zhenzhen1,2; Yu, Cong1,2 |
| 刊名 | Analytical sciences
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 32期号:10页码:1035-1037 |
| 关键词 | Qds Target dna Fluorescence Deoxyribonuclease i Fret |
| ISSN号 | 0910-6340 |
| 通讯作者 | Yu, cong(congyu@ciac.ac.cn) |
| 英文摘要 | A black-hole quencher (bhq-2) labeled dna (q-dna) with a phosphorothioate backbone was covalently conjugated to the cdte qds during the qds synthesis procedure. the hairpin structure of q-dna shortened the distance of the cdte qds and the bhq-2 group, which resulted in fluorescence quenching of the qds. the addition of target dna or deoxyribonuclease i (dnase i) could move the bhq-2 group away from the qds. as a result, the fluorescence of the cdte qds recovered. this work provides a new way for target dna and dnase i detection. |
| WOS关键词 | SEMICONDUCTOR QUANTUM DOTS ; PLATFORM |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000386423300001 |
| 出版者 | JAPAN SOC ANALYTICAL CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375010 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, Cong |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, 5625 Renmin St, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Tianjin Univ, Sch Mat Sci & Engn, Tianjin 300072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qingfeng,Li, Juanmin,Zhang, Yunyi,et al. One-pot synthesis of quencher labeled hairpin dna-cdte qds conjugate for target dna and deoxyribonuclease i detection[J]. Analytical sciences,2016,32(10):1035-1037. |
| APA | Zhang, Qingfeng,Li, Juanmin,Zhang, Yunyi,Hu, Zhenzhen,&Yu, Cong.(2016).One-pot synthesis of quencher labeled hairpin dna-cdte qds conjugate for target dna and deoxyribonuclease i detection.Analytical sciences,32(10),1035-1037. |
| MLA | Zhang, Qingfeng,et al."One-pot synthesis of quencher labeled hairpin dna-cdte qds conjugate for target dna and deoxyribonuclease i detection".Analytical sciences 32.10(2016):1035-1037. |
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来源：中国科学院大学
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