Protective effects of arginine on saccharomyces cerevisiae against ethanol stress
文献类型：期刊论文
| 作者 | Cheng, Yanfei1; Du, Zhaoli1,2,3; Zhu, Hui1,2; Guo, Xuena1; He, Xiuping1 |
| 刊名 | Scientific reports
 |
| 出版日期 | 2016-08-10 |
| 卷号 | 6页码:12 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep31311 |
| 通讯作者 | He, xiuping(hexp@im.ac.cn) |
| 英文摘要 | Yeast cells are challenged by various environmental stresses in the process of industrial fermentation. as the currently main organism for bio-ethanol production, saccharomyces cerevisiae suffers from ethanol stress. some amino acids have been reported to be related to yeast tolerance to stresses. here the relationship between arginine and yeast response to ethanol stress was investigated. marked inhibitions of ethanol on cell growth, expression of genes involved in arginine biosynthesis and intracellular accumulation of arginine were observed. furthermore, extracellular addition of arginine can abate the ethanol damage largely. to further confirm the protective effects of arginine on yeast cells, yeast strains with different levels of arginine content were constructed by overexpression of arg4 involved in arginine biosynthesis or car1 encoding arginase. intracellular arginine was increased by 18.9% or 13.1% respectively by overexpression of arg4 or disruption of car1, which enhanced yeast tolerance to ethanol stress. moreover, a 41.1% decrease of intracellular arginine was observed in car1 overexpressing strain, which made yeast cells keenly sensitive to ethanol. further investigations indicated that arginine protected yeast cells from ethanol damage by maintaining the integrity of cell wall and cytoplasma membrane, stabilizing the morphology and function of organellae due to low ros generation. |
| WOS关键词 | HIGH-TEMPERATURE ; YEAST ; TOLERANCE ; PROLINE ; GENES ; AGGREGATION ; IDENTIFICATION ; STABILIZATION ; SUPPRESSION ; METABOLISM |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000381107300001 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375129 |
| 专题 | 中国科学院大学 |
| 通讯作者 | He, Xiuping |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Biomarker Technol CO LTD, 12 Fuqianjie, Beijing 101300, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Yanfei,Du, Zhaoli,Zhu, Hui,et al. Protective effects of arginine on saccharomyces cerevisiae against ethanol stress[J]. Scientific reports,2016,6:12. |
| APA | Cheng, Yanfei,Du, Zhaoli,Zhu, Hui,Guo, Xuena,&He, Xiuping.(2016).Protective effects of arginine on saccharomyces cerevisiae against ethanol stress.Scientific reports,6,12. |
| MLA | Cheng, Yanfei,et al."Protective effects of arginine on saccharomyces cerevisiae against ethanol stress".Scientific reports 6(2016):12. |
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来源：中国科学院大学
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