Insight of in vitro small-interfering rna release from chitosan nanoparticles under enzymolysis with forster resonance energy transfer analysis
文献类型：期刊论文
| 作者 | Zhang, Demeng1,2; Song, Yizhe1,2; Wang, Yu1,2; Liu, Xiudong3; Yu, Weiting1; Ma, Xiaojun1 |
| 刊名 | Journal of pharmaceutical sciences
 |
| 出版日期 | 2016 |
| 卷号 | 105期号:1页码:301-307 |
| 关键词 | Nonviral gene delivery Rnai Sirna Chitosan Nanoparticles Nanotechnology |
| ISSN号 | 0022-3549 |
| DOI | 10.1002/jps.24698 |
| 通讯作者 | Yu, weiting(yuwt@dicp.ac.cn) |
| 英文摘要 | Small-interfering rna (sirna)-mediated gene silencing with the aid of chitosan (cs)-based carriers has shown efficient and reliable outcome in vitro, but the gene silencing efficiency in vivo is still limited. it is of great importance to balance the protection and release of sirna from nanoparticles (nps) so as to achieve high efficiency. however, sirna release profile from cs/sirna nps has been rarely concerned. here, forster resonance energy transfer technique was adopted for in vitro investigation of sirna release from cs nps in lysozyme-contained buffer. the results clearly showed that sirna molecules experienced a fast and short release phase under lysozyme competition to both cs and sirna, and then a slow and long release under lysozyme degradation on cs. moreover, lysozyme competition played more important role than enzymolysis on trigging sirna release. this preliminary study of sirna release is the first step to get insight of in vivo sirna release mechanism from cs/sirna nps, which will be helpful to adjust the design of cs/sirna nps for balancing the protection and release of sirna molecules. (c) 2016 american pharmacists association (r). published by elsevier inc. all rights reserved. |
| WOS关键词 | SIRNA DELIVERY ; INTRACELLULAR TRAFFICKING ; GENE DELIVERY ; LYSOZYME ; CHITIN ; THERAPEUTICS ; FORMULATION ; POLYPLEXES ; SYSTEMS ; PROBES |
| WOS研究方向 | Pharmacology & Pharmacy ; Chemistry |
| WOS类目 | Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000381768000037 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375136 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, Weiting |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Demeng,Song, Yizhe,Wang, Yu,et al. Insight of in vitro small-interfering rna release from chitosan nanoparticles under enzymolysis with forster resonance energy transfer analysis[J]. Journal of pharmaceutical sciences,2016,105(1):301-307. |
| APA | Zhang, Demeng,Song, Yizhe,Wang, Yu,Liu, Xiudong,Yu, Weiting,&Ma, Xiaojun.(2016).Insight of in vitro small-interfering rna release from chitosan nanoparticles under enzymolysis with forster resonance energy transfer analysis.Journal of pharmaceutical sciences,105(1),301-307. |
| MLA | Zhang, Demeng,et al."Insight of in vitro small-interfering rna release from chitosan nanoparticles under enzymolysis with forster resonance energy transfer analysis".Journal of pharmaceutical sciences 105.1(2016):301-307. |
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来源：中国科学院大学
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