A new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins
文献类型：期刊论文
| 作者 | Chen, Chaohui1; Liu, Yufei1; Zheng, Zhenhua2; Zhou, Guohua1; Ji, Xinghu1; Wang, Hanzhong2; He, Zhike1 |
| 刊名 | Analytica chimica acta
 |
| 出版日期 | 2015-06-23 |
| 卷号 | 880页码:1-7 |
| 关键词 | Assembly Colorimetric Amplification Protein detection |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2015.05.010 |
| 通讯作者 | He, zhike(zhkhe@whu.edu.cn) |
| 英文摘要 | An amplified colorimetric method has been developed for the detection of protein and cancer cells based on the assembly of nucleic acids and proteins for the first time. in this process, the assembly of nucleic acids was triggered by a biotinylated dna strand after a sandwich immunoreaction. the biotinylated dna strand and sandwich immunocomplex were connected by streptavidin. then, the assembly of biotinylated bovine serum albumin (biotin-bsa) and streptavidin-horseradish peroxidase (sa-hrp) occurred at a node of the assembled products of nucleic acids through the biotin-streptavidin reaction. under the catalysis of horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine (tmb) was oxidized by h2o2 and the oxidized product was analyzed by its uv-vis absorbance signal and sensitive colorimetric detection. this colorimetric sensor could not only achieve the quantitative determination of protein by uv-vis absorbance but could also be applied for semiquantitative determination by digital visualization. using alpha-fetoprotein (afp) as the model target, this proposed colorimetric method showed a wide linear range from 5 pg/ml to 1 ng/ml with a detection limit of 1.95 pg/ml by the instrument, and even 5 pg/ml target protein could be distinguished simply by the naked eye. this approach was then expanded to detect cancer cells based on the recognition of folic acid receptors that were over-expressed on the cancer cells by folic acid-tethered dna. more importantly, this strategy can be further used as a universal colorimetric method for the determination of viruses or other proteins by changing the corresponding antibodies. (c) 2015 elsevier b.v. all rights reserved. |
| WOS关键词 | HYBRIDIZATION CHAIN-REACTION ; ROLLING CIRCLE AMPLIFICATION ; ELECTROCHEMICAL PROXIMITY ASSAY ; SIGNAL AMPLIFICATION ; DNA ; NANOPARTICLES ; COMPLEX ; STRATEGY ; APTAMERS ; ENZYME |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000356234800001 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375172 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | He, Zhike |
| 作者单位 | 1.Wuhan Univ, Coll Chem & Mol Sci, Key Lab Analyt Chem Biol & Med, Minist Educ, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Chaohui,Liu, Yufei,Zheng, Zhenhua,et al. A new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins[J]. Analytica chimica acta,2015,880:1-7. |
| APA | Chen, Chaohui.,Liu, Yufei.,Zheng, Zhenhua.,Zhou, Guohua.,Ji, Xinghu.,...&He, Zhike.(2015).A new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins.Analytica chimica acta,880,1-7. |
| MLA | Chen, Chaohui,et al."A new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins".Analytica chimica acta 880(2015):1-7. |
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来源：武汉病毒研究所
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